Table of Contents: “... Liver Injury -- In Vitro Porcine (Explant) Colon Culture -- Bioencapsulation of Oocytes and Granulosa Cells -- Culturing and Differentiation of Patient-Derived Ectocervical Epithelial Stem Cells using Air-Liquid Interphase and Matrigel Scaffold -- Ovine...”

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Table of Contents: “... Macrophages in Adult Zebrafish -- Genetic and Immunohistochemistry Tools to Visualize Rat Macrophages In Situ -- Phenotyping of Macrophages in Human Immune System Mice -- Fate-Mapping of Yolk Sac-Derived Macrophages -- Fate-Mapping of Hematopoietic Stem Cell...”

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Table of Contents: “...Isolation and Purification of Viable PGCs from Mouse Embryos -- Purification and Transfection Methods of Chicken Primordial Germ Cells -- Isolation and In Vitro Propagation of Human Spermatogonial Stem Cells -- Purification by STA-PUT Technique of Male...”

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Table of Contents: “... to Study Human Placental Development and Function -- A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening -- Three-Dimensional In Vitro Human Placental Organoids from Mononuclear Villous Trophoblasts or Trophoblast Stem...”

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Table of Contents: “... -- Genomic Engineering of Induced Pluripotent Stem Cell-Derived Cardiomyocytes -- The GENTIL Method for Isolation of Human Adult Cardiomyocytes from Cryopreserved Tissue for Proteomic Analyses -- Motility Assay to Probe the Calcium Sensitivity of Myosin...”

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Table of Contents: “... Organisms -- Whole Mount Immunofluorescence Staining to Visualize Cell Cycle Progression in Mouse Oocyte Meiosis -- Imaging and Analysis of Drosophila Neural Stem Cell Asymmetric Division -- Cell Cycle Mapping Using Multiplexed Immunofluorescence -- Investigating...”

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Table of Contents: “...Isolation of Human Adipose-Derived Stem Cells -- Isolation of Huma n Adipose-Derived Stem Cells from Lipoaspirates -- Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry -- Soft Tissue Reconstruction...”

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Table of Contents: “...Generation of Human Blastoids from Naïve Pluripotent Stem Cells -- Human Pre-Gastrulation Embryo Culture in 3D Condition -- Protocol for the Generation of Human EPS-Blastoids Using a Three-Dimensional Two-Step Induction System -- Generation of Human...”

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Table of Contents: “... Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells -- Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo -- Angiogenesis Assays for the Analysis of CCN Proteins -- Using...”

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Table of Contents: “... in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts -- Immunofluorescence Microscopy of the Mammalian Golgi Apparatus -- Studying the Organization of the Golgi by Super-Resolution Microscopy -- Super-Resolution Live Imaging...”

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Table of Contents: “... that Affect PARP1 Activity In Vivo -- Generating PARP Knockout D. Melanogaster with CRISPR/Cas9 System -- TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice -- Cell-Based Screening for New PARP Inhibitors Utilizing PARG Mutated Mouse Embryonic Stem Cells...”

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Table of Contents: “... Bilayers to Probe the Mechanosensitivity of Signaling Receptors -- Monitoring Mechano-Regulation of Gene Expression by RNA Sequencing -- Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK...”

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Table of Contents: “... Patients -- Serum-Free Production of Human Stem Cell-Derived Liver Spheres for Cancer Metastasis Research -- A Self-Assembly Method for Creating Vascularized Tumor Explants Using Biomaterials for 3D Culture -- A Step-by-Step Methodological Guide for Developing...”

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Table of Contents: “...Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update -- Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells -- Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells...”

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Table of Contents: “... Gun DiOlistic Labeling of Retinal Ganglion Cells -- Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells -- Laser-Induced Ocular Hypertension in a Mouse Model of Glaucoma -- Silicone Oil-Induced Ocular Hypertension...”

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Table of Contents: “...-Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry -- An RNA/DNA-Based Flow Cytometry Approach for Isolating Slow-Cycling Stem Cells -- ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian...”

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Table of Contents: “...Dissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens -- Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions -- Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish -- Transient...”

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Table of Contents: “... -- Optogenetic Control of TGF-β Signaling -- Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-β Signaling in Mouse Embryonic Stem Cells -- Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network -- Discrete Logic Modeling...”

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Table of Contents: “...Chapter 1. Introduction -- Chapter 2. Cell Culture -- Chapter 3. Cell Metabolism -- Chapter 4. Cell Imaging -- Chapter 5. Stem Cells -- Chapter 6. Biomaterial Surfaces -- Chapter 7. Focal Adhesion -- Chapter 8. Contact Guidance and Cell Patterning...”

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Table of Contents: “...A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment -- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells -- Organoid Assay of Human Cancer Stem Cells...”

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