Single Domain Antibodies [E-Book]: Methods and Protocols / edited by Dirk Saerens, Serge Muyldermans.
The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome e...
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Full text |
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Personal Name(s): | Muyldermans, Serge. |
Saerens, Dirk. | |
Imprint: |
Totowa, NJ :
Humana Press,
2012
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Physical Description: |
XV, 580 p. 89 illus., 35 illus. in color. digital. |
Note: |
englisch |
ISBN: |
9781617799686 |
DOI: |
10.1007/978-1-61779-968-6 |
Series Title: |
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Methods in molecular biology, methods and protocols ;
911 |
Subject (LOC): |
- From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small
- Introduction to Heavy Chain Antibodies and Derived Nanobodies
- Overview and Discovery of IgNARs and Generation of VNARs
- Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies
- Preparation of a Naïve Library of Camelid Single Domain Antibodies
- Selection by Phage Display of Single Domain Antibodies Specific to Antigens in Their Native Conformation
- Semi-Automated Panning of Naive Camelidae Libraries and Selection of Single-Domain Antibodies Against Peptide Antigens
- Pichia Surface Display: A Tool for Screening Single Domain Antibodies
- Bacterial Two Hybrid: A Versatile One-Step Intracellular Selection Method
- Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies
- Selection of Functional Single Domain Antibody Fragments for Interfering with Protein-Protein Interactions Inside Cells: A “One Plasmid” Mammalian Two-Hybrid System
- Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization
- Selection of VHHs Under Application Conditions
- Isolation and Characterization of Clostridium difficile Toxin-Specific Single-Domain Antibodies
- Selection of VHH Antibody Fragments that Recognize Different Aβ Depositions Using Complex Immune Libraries
- Expression of Single-Domain Antibodies in Bacterial Systems
- Expression of VHHs in S. cerevisiae
- Stable Expression of Chimeric Heavy Chain Antibodies in CHO Cells
- Production of Camel-Like Antibodies in Plants
- Selecting and Purifying Autonomous Human Variable Heavy (VH) Domains
- Solubility and Stability Engineering of Human VH Domains
- Improvement of Proteolytic Stability through In Silico Engineering
- Selection of Human VH Single Domains with Improved Biophysical Properties by Phage Display
- Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction
- Characterization of Single-Domain Antibodies with an Engineered Disulfide Bond
- Affinity Maturation of Single-Domain Antibodies by Yeast Surface Display
- Multivalent Display of Single-Domain Antibodies
- Methods for Determining the PK Parameters of AlbudAbs and of Long Serum Half Life Drugs Made Using the AlbudAb Technology
- Fluorescent Protein Specific Nanotraps to Study Protein-Protein Interactions and Histone-Tail Peptide Binding
- Site-Specific Labeling of His-Tagged Nanobodies with 99mTc: A Practical Guide
- Nanobody-Based Chromatin Immunoprecipitation
- User-Friendly Expression Plasmids Enable the Fusion of VHHs to Application-Specific Tags
- Application of Single Domain Antibodies in Tumor Histochemistry
- Nanobodies as Structural Probes of Protein Misfolding and Fibril Formation
- Molecular Imaging Using Nanobodies: A Case Study
- Case Study on Live Cell Apoptosis-Assay Using Lamin-Chromobody Cell-Lines for High-Content Analysis..