Mass Spectrometry of Glycoproteins [E-Book] : Methods and Protocols / edited by Jennifer J. Kohler, Steven M. Patrie.
Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the...
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Full text |
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Personal Name(s): | Kohler, Jennifer J. |
Patrie, Steven M. | |
Imprint: |
Totowa, NJ :
Humana Press,
2013
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Physical Description: |
XI, 339 p. 90 illus., 12 illus. in color. digital. |
Note: |
englisch |
ISBN: |
9781627031462 |
DOI: |
10.1007/978-1-62703-146-2 |
Series Title: |
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Methods in molecular biology, methods and protocols ;
951 |
Subject (LOC): |
Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry. |