Transgenic Mouse [E-Book] : Methods and Protocols / edited by Melissa A. Larson.
This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer...
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Full text |
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Personal Name(s): | Larson, Melissa A., editor |
Edition: |
1st edition 2020. |
Imprint: |
New York, NY :
Humana Press,
2020
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Physical Description: |
X, 236 pages 45 illustrations, 27 illustrations in color (online resource) |
Note: |
englisch |
ISBN: |
9781493998371 |
DOI: |
10.1007/978-1-4939-9837-1 |
Series Title: |
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Methods in Molecular Biology ;
2066 |
Subject (ZB): | |
Subject (LOC): | |
Classification: |
- The History of Transgenesis
- Pronuclear Microinjection of One-Cell Embryos
- Integrase-Mediated Targeted Transgenics through Pronuclear Microinjection
- In Vivo Validation of CRISPR Reagents in Pre-Implantation Mouse Embryos
- Targeted Mutations in the Mouse via Embryonic Stem Cells
- Blastocyst Microinjection with Embryonic Stem Cells
- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos
- Embryo Transfer Surgery
- Non-Surgical Embryo Transfer Protocol for use with the NSET Device
- Nuclear Transfer and Cloning
- Sperm-Mediated Genetic Modifications
- Genotyping Genetically Modified (GM) Mice
- Nomenclature: Naming Your Gene-Modified Mouse
- Breeding Strategies for Genetically Modified Mice
- Strategies for Behaviorally Phenotyping the Transgenic Mouse
- Cryobanking and Recovery of Genetically Modified Mice
- Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques
- Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells using Sendai Virus.