This title appears in the Scientific Report :
2011
Please use the identifier:
http://dx.doi.org/10.1016/j.jbiozec2010.07.004 in citations.
Citrate synthase in Corynebacterium glutamicum is encoded by two gltA transcripts which are controlled by RamA, RamB and GlxR
Citrate synthase in Corynebacterium glutamicum is encoded by two gltA transcripts which are controlled by RamA, RamB and GlxR
Citrate synthase (CS) is located at a major branch point in the metabolism and is required for both tricarboxylic acid and glyoxylic acid cycle activity. Here we show that the CS gene gltA of Corynebacterium glutamicum is monocistronic, but that two transcripts are formed with their transcript initi...
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Personal Name(s): | van Ooyen, J. |
---|---|
Emer, D. / Bussmann, M. / Bott, M. / Eikmanns, B.J. / Eggeling, L. | |
Contributing Institute: |
Biotechnologie 1; IBT-1 |
Published in: | Journal of biotechnology, 154 (2011) |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2011
|
DOI: |
10.1016/j.jbiozec2010.07.004 |
Document Type: |
Journal Article |
Research Program: |
Biotechnologie |
Series Title: |
Journal of Biotechnology
154 |
Subject (ZB): | |
Publikationsportal JuSER |
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520 | |a Citrate synthase (CS) is located at a major branch point in the metabolism and is required for both tricarboxylic acid and glyoxylic acid cycle activity. Here we show that the CS gene gltA of Corynebacterium glutamicum is monocistronic, but that two transcripts are formed with their transcript initiation sites located 121 bp and 357 bp upstream of the translational start codon, respectively. Northern blot analyses revealed that during growth on acetate the short transcript prevails, whereas during growth on glucose the long transcript is dominant. Further Northern blots, reporter gene fusions, and CS activity measurements in mutants devoid of the transcriptional regulators RamA and RamB or with the global regulator GlxR overexpressed revealed a complex involvement of these regulators in gltA transcription. This was confirmed by demonstrating the direct interaction of isolated RamA, RamB and GlxR proteins with specific gltA promoter regions in vitro. The combined analyses point to an elaborate control of gltA transcript formation, which is possibly required as a dedicated mechanism to balance the total CS activity according to the physiological requirements. (C) 2010 Elsevier B. V. All rights reserved. | ||
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500 | |a The support provided by the German Ministry of Education and Research (BMBF) to LE and MHB (grant 0313704 "SysMAP") and to BJE (grant 0313704 "SysMAP" and grant 0313805G "GenoMik-Plus") is gratefully acknowledged. | ||
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