This title appears in the Scientific Report :
2010
Please use the identifier:
http://dx.doi.org/10.1099/mic.0.040667-0 in citations.
L-Glutamine as nitrogen source for glutamicum: derepression of the AmtR regulon and implications for nitrogen sensing
L-Glutamine as nitrogen source for glutamicum: derepression of the AmtR regulon and implications for nitrogen sensing
Corynebacterium glutamicum, a Gram-positive soil bacterium employed in the industrial production of various amino acids, is able to use a number of different nitrogen sources, such as ammonium, urea or creatinine. This study shows that l-glutamine serves as an excellent nitrogen source for C. glutam...
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Personal Name(s): | Rehm, N. |
---|---|
Georgi, T. / Hiery, E. / Degner, U. / Schmiedl, A. / Burkovski, A. / Bott, M. | |
Contributing Institute: |
Biotechnologie 1; IBT-1 |
Published in: | Microbiology, 156 (2010) |
Imprint: |
Reading
Soc.
2010
|
DOI: |
10.1099/mic.0.040667-0 |
PubMed ID: |
20656783 |
Document Type: |
Journal Article |
Research Program: |
Biotechnologie |
Series Title: |
Microbiology
156 |
Subject (ZB): | |
Publikationsportal JuSER |
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100 | 1 | |a Rehm, N. |b 0 |0 P:(DE-HGF)0 | |
245 | |a L-Glutamine as nitrogen source for glutamicum: derepression of the AmtR regulon and implications for nitrogen sensing | ||
260 | |a Reading |b Soc. |c 2010 | ||
440 | 0 | |a Microbiology |x 1350-0872 |0 4340 |y 3180 - 3193 |v 156 | |
500 | |a The authors wish to thank Lars Nolden (Cologne) for providing strain LN Delta gltBD, Julia Frunzke (Julich) for testing the pH profiles and Tino Polen (Julich) for deposition of the microarray data in the GEO database. This work was supported by the Bundesministerium fur Bildung und Forschung (BMBF) within the GenoMik-Plus programme. | ||
520 | |a Corynebacterium glutamicum, a Gram-positive soil bacterium employed in the industrial production of various amino acids, is able to use a number of different nitrogen sources, such as ammonium, urea or creatinine. This study shows that l-glutamine serves as an excellent nitrogen source for C. glutamicum and allows similar growth rates in glucose minimal medium to those in ammonium. A transcriptome comparison revealed that the nitrogen starvation response was elicited when glutamine served as the sole nitrogen source, meaning that the target genes of the global nitrogen regulator AmtR were derepressed. Subsequent growth experiments with a variety of mutants defective in nitrogen metabolism showed that glutamate synthase is crucial for glutamine utilization, while a putative glutaminase is dispensable under the experimental conditions used. The gltBD operon encoding the glutamate synthase is a member of the AmtR regulon. The observation that the nitrogen starvation response was elicited at high intracellular l-glutamine levels has implications for nitrogen sensing. In contrast with other Gram-positive and Gram-negative bacteria such as Bacillus subtilis, Salmonella enterica serovar Typhimurium and Klebsiella pneumoniae, a drop in glutamine concentration obviously does not serve as a nitrogen starvation signal in C. glutamicum. | ||
588 | |a Dataset connected to Web of Science, Pubmed | ||
650 | 2 | |2 MeSH |a Bacterial Proteins: genetics | |
650 | 2 | |2 MeSH |a Bacterial Proteins: metabolism | |
650 | 2 | |2 MeSH |a Corynebacterium glutamicum: genetics | |
650 | 2 | |2 MeSH |a Corynebacterium glutamicum: growth & development | |
650 | 2 | |2 MeSH |a Corynebacterium glutamicum: metabolism | |
650 | 2 | |2 MeSH |a Culture Media | |
650 | 2 | |2 MeSH |a Gene Expression Profiling | |
650 | 2 | |2 MeSH |a Gene Expression Regulation, Bacterial | |
650 | 2 | |2 MeSH |a Glutamate Synthase: metabolism | |
650 | 2 | |2 MeSH |a Glutamine: metabolism | |
650 | 2 | |2 MeSH |a Mutation | |
650 | 2 | |2 MeSH |a Nitrogen: metabolism | |
650 | 2 | |2 MeSH |a Oligonucleotide Array Sequence Analysis | |
650 | 2 | |2 MeSH |a Quaternary Ammonium Compounds: metabolism | |
650 | 2 | |2 MeSH |a RNA, Bacterial: genetics | |
650 | 2 | |2 MeSH |a Regulon | |
650 | 2 | |2 MeSH |a Repressor Proteins: genetics | |
650 | 2 | |2 MeSH |a Repressor Proteins: metabolism | |
650 | 7 | |0 0 |2 NLM Chemicals |a AmtR protein, Corynebacterium glutamicum | |
650 | 7 | |0 0 |2 NLM Chemicals |a Bacterial Proteins | |
650 | 7 | |0 0 |2 NLM Chemicals |a Culture Media | |
650 | 7 | |0 0 |2 NLM Chemicals |a Quaternary Ammonium Compounds | |
650 | 7 | |0 0 |2 NLM Chemicals |a RNA, Bacterial | |
650 | 7 | |0 0 |2 NLM Chemicals |a Repressor Proteins | |
650 | 7 | |0 56-85-9 |2 NLM Chemicals |a Glutamine | |
650 | 7 | |0 7727-37-9 |2 NLM Chemicals |a Nitrogen | |
650 | 7 | |0 EC 1.4.1.13 |2 NLM Chemicals |a Glutamate Synthase | |
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