This title appears in the Scientific Report :
2014
Please use the identifier:
http://dx.doi.org/10.1016/j.biomaterials.2014.05.027 in citations.
The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes
The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes
Cardiomyocytes (CMs) from induced pluripotent stem (iPS) cells mark an important achievement in the development of in vitro pharmacological, toxicological and developmental assays and in the establishment of protocols for cardiac cell replacement therapy. Using CMs generated from murine embryonic st...
Saved in:
Personal Name(s): | Heras-Bautista, Carlos O. (Corresponding Author) |
---|---|
Katsen-Globa, Alisa / Schloerer, Nils E. / Dieluweit, Sabine / Abd El Aziz, Osama M. / Peinkofer, Gabriel / Attia, Wael A. / Khalil, Markus / Brockmeier, Konrad / Hescheler, Jürgen / Pfannkuche, Kurt | |
Contributing Institute: |
Biomechanik; ICS-7 |
Published in: | Biomaterials, 35 (2014) 26, S. 7374 - 7385 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2014
|
PubMed ID: |
24889032 |
DOI: |
10.1016/j.biomaterials.2014.05.027 |
Document Type: |
Journal Article |
Research Program: |
Physics of the Cell |
Publikationsportal JuSER |
LEADER | 05581nam a2200853 a 4500 | ||
---|---|---|---|
001 | 154975 | ||
005 | 20210129214003.0 | ||
024 | 7 | |a 10.1016/j.biomaterials.2014.05.027 |2 doi | |
024 | 7 | |a 0142-9612 |2 ISSN | |
024 | 7 | |a 1878-5905 |2 ISSN | |
024 | 7 | |a WOS:000339035000012 |2 WOS | |
024 | 7 | |a altmetric:2425631 |2 altmetric | |
024 | 7 | |a pmid:24889032 |2 pmid | |
037 | |a FZJ-2014-04182 | ||
082 | |a 570 | ||
100 | 1 | |a Heras-Bautista, Carlos O. |0 P:(DE-HGF)0 |b 0 |e Corresponding Author | |
245 | |a The influence of physiological matrix conditions on permanent culture of induced pluripotent stem cell-derived cardiomyocytes | ||
260 | |a Amsterdam [u.a.] |c 2014 |b Elsevier Science | ||
520 | |a Cardiomyocytes (CMs) from induced pluripotent stem (iPS) cells mark an important achievement in the development of in vitro pharmacological, toxicological and developmental assays and in the establishment of protocols for cardiac cell replacement therapy. Using CMs generated from murine embryonic stem cells and iPS cells we found increased cell–matrix interaction and more matured embryoid body (EB) structures in iPS cell-derived EBs. However, neither suspension-culture in form of purified cardiac clusters nor adherence-culture on traditional cell culture plastic allowed for extended culture of CMs. CMs grown for five weeks on polystyrene exhibit signs of massive mechanical stress as indicated by α-smooth muscle actin expression and loss of sarcomere integrity. Hydrogels from polyacrylamide allow adapting of the matrix stiffness to that of cardiac tissue. We were able to eliminate the bottleneck of low cell adhesion using 2,5-Dioxopyrrolidin-1-yl-6-acrylamidohexanoate as a crosslinker to immobilize matrix proteins on the gels surface. Finally we present an easy method to generate polyacrylamide gels with a physiological Young's modulus of 55 kPa and defined surface ligand, facilitating the culture of murine and human iPS-CMs, removing excess mechanical stresses and reducing the risk of tissue culture artifacts exerted by stiff substrates. | ||
588 | |a Dataset connected to CrossRef, juser.fz-juelich.de | ||
700 | 1 | |a Katsen-Globa, Alisa |0 P:(DE-HGF)0 |b 1 | |
700 | 1 | |a Schloerer, Nils E. |0 P:(DE-HGF)0 |b 2 | |
700 | 1 | |a Dieluweit, Sabine |0 P:(DE-Juel1)128807 |b 3 |u fzj | |
700 | 1 | |a Abd El Aziz, Osama M. |0 P:(DE-HGF)0 |b 4 | |
700 | 1 | |a Peinkofer, Gabriel |0 P:(DE-HGF)0 |b 5 | |
700 | 1 | |a Attia, Wael A. |0 P:(DE-HGF)0 |b 6 | |
700 | 1 | |a Khalil, Markus |0 P:(DE-HGF)0 |b 7 | |
700 | 1 | |a Brockmeier, Konrad |0 P:(DE-HGF)0 |b 8 | |
700 | 1 | |a Hescheler, Jürgen |0 P:(DE-HGF)0 |b 9 | |
700 | 1 | |a Pfannkuche, Kurt |0 P:(DE-HGF)0 |b 10 | |
773 | |a 10.1016/j.biomaterials.2014.05.027 |g Vol. 35, no. 26, p. 7374 - 7385 |0 PERI:(DE-600)2004010-6 |n 26 |p 7374 - 7385 |t Biomaterials |v 35 |y 2014 |x 0142-9612 | ||
856 | 4 | |u http://juser.fz-juelich.de/record/154975/files/FZJ-2014-04182.pdf |y Restricted | |
909 | C | O | |o oai:juser.fz-juelich.de:154975 |p VDB |
910 | 1 | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)128807 | |
913 | 2 | |a DE-HGF |b Key Technologies |l BioSoft Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-552 |2 G:(DE-HGF)POF3-500 |v Engineering Cell Function |x 0 | |
913 | 1 | |a DE-HGF |b Schlüsseltechnologien |1 G:(DE-HGF)POF2-450 |0 G:(DE-HGF)POF2-453 |2 G:(DE-HGF)POF2-400 |v Physics of the Cell |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF2 |l BioSoft | |
914 | 1 | |y 2014 | |
915 | |a JCR/ISI refereed |0 StatID:(DE-HGF)0010 |2 StatID | ||
915 | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID | ||
915 | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index | ||
915 | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database | ||
915 | |a Nationallizenz |0 StatID:(DE-HGF)0420 |2 StatID | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews | ||
980 | |a journal | ||
980 | |a VDB | ||
980 | |a I:(DE-Juel1)ICS-7-20110106 | ||
980 | |a UNRESTRICTED | ||
536 | |a Physics of the Cell |0 G:(DE-HGF)POF2-453 |c POF2-453 |f POF II |x 0 | ||
336 | |a ARTICLE |2 BibTeX | ||
336 | |a Nanopartikel unedler Metalle (Mg0, Al0, Gd0, Sm0) |0 0 |2 EndNote | ||
336 | |a Output Types/Journal article |2 DataCite | ||
336 | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1407767291_30524 |2 PUB:(DE-HGF) | ||
336 | |a article |2 DRIVER | ||
336 | |a JOURNAL_ARTICLE |2 ORCID | ||
981 | |a I:(DE-Juel1)IBI-2-20200312 | ||
920 | |l yes | ||
920 | |k Biomechanik; ICS-7 |0 I:(DE-Juel1)ICS-7-20110106 |l Biomechanik |x 0 | ||
991 | |a Hescheler, Jürgen |0 P:(DE-HGF)0 |b 9 | ||
991 | |a Brockmeier, Konrad |0 P:(DE-HGF)0 |b 8 | ||
991 | |a Khalil, Markus |0 P:(DE-HGF)0 |b 7 | ||
991 | |a Attia, Wael A. |0 P:(DE-HGF)0 |b 6 | ||
990 | |a Heras-Bautista, Carlos O. |0 P:(DE-HGF)0 |b 0 |e Corresponding Author | ||
991 | |a Peinkofer, Gabriel |0 P:(DE-HGF)0 |b 5 | ||
991 | |a Pfannkuche, Kurt |0 P:(DE-HGF)0 |b 10 | ||
991 | |a Abd El Aziz, Osama M. |0 P:(DE-HGF)0 |b 4 | ||
991 | |a Dieluweit, Sabine |0 P:(DE-Juel1)128807 |b 3 |u fzj | ||
991 | |a Schloerer, Nils E. |0 P:(DE-HGF)0 |b 2 | ||
991 | |a Katsen-Globa, Alisa |0 P:(DE-HGF)0 |b 1 |