This title appears in the Scientific Report :
2015
Please use the identifier:
http://dx.doi.org/10.1016/j.proeng.2015.08.702 in citations.
Please use the identifier: http://hdl.handle.net/2128/9781 in citations.
Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes
Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes
An enzyme-based multi-parameter sensor chip for the simultaneous measurement of formate, D- and L-lactate is presented. Thereby, the combination of a diaphorase (DIA) from Clostridium kluyveri with different NAD+-dependent dehydrogenases enables the specific recognition of each compound. The amperom...
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Personal Name(s): | Pilas, J. |
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Mariano, K. / Keusgen, M. / Selmer, T. / Schöning, M. J. (Corresponding author) | |
Contributing Institute: |
Bioelektronik; PGI-8 |
Published in: | Procedia engineering, 120 (2015) S. 532 - 535 |
Imprint: |
Amsterdam [u.a.]
Elsevier
2015
|
Physical Description: |
532 - 535 |
DOI: |
10.1016/j.proeng.2015.08.702 |
Conference: | eurosensors 2015, Freiburg (Germany), 2015-09-06 - 2015-09-09 |
Document Type: |
Contribution to a conference proceedings Journal Article |
Research Program: |
Controlling Configuration-Based Phenomena |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://hdl.handle.net/2128/9781 in citations.
An enzyme-based multi-parameter sensor chip for the simultaneous measurement of formate, D- and L-lactate is presented. Thereby, the combination of a diaphorase (DIA) from Clostridium kluyveri with different NAD+-dependent dehydrogenases enables the specific recognition of each compound. The amperometric detection is performed via monitoring of the oxidation current of enzymatically produced hexacyanoferrate(II) at +300 mV vs. Ag/AgCl. Chemical cross-linking with glutaraldehyde is used for enzyme immobilization. Thereby, the sensor signal is investigated at different concentrations of the cross-linker in the enzyme membrane. The obtained results indicate that by optimization of the immobilization matrix, the sensor performance can be improved. |