This title appears in the Scientific Report : 2003 

Flow injection amperometric detection of OP nerve agents based on organophosphorus-hydrolase biosensor detector
Wang, J.
Krause, R. / Block, K. / Musameh, M. / Mulchandani, A. / Schöning, M. J.
Institut für Bio- und Chemosensoren; ISG-2
Biosensors and bioelectronics, 18 (2003) S. 255 - 260
Amsterdam [u.a.] Elsevier Science 2003
255 - 260
10.1016/S0956-5663(02)00178-1
Journal Article
Materialien, Prozesse und Bauelemente für die Mikro- und Nanoelektronik
J
Please use the identifier: http://dx.doi.org/10.1016/S0956-5663(02)00178-1 in citations.
A flow-injection system with an organophosphorus-hydrolase (OPH)-biosensor detector has been developed and characterized for the rapid detection of organophosphorus (OP) nerve agents. The enzyme was immobilized onto a thin-film gold detector through a cystamine-glutaraldehyde coupling. Factors influencing the performance were optimized. The resulting flow system offered a fast, sensitive, selective, and stable response. The peak current increased linearly with the concentration of paraoxon and methyl parathion over the 1-10 muM range (sensitivity, 2.29 and 1.04 nA/muM, respectively). The OPH-biosensor flow injection systems offered low detection limits (e.g. 0.1 muM paraoxon), along with a good precision (R.S.D. of 3.6% for 20 successive injections of a 1.0 muM paraoxon solution). The OPH-biosensor flow detector offers great promise for rapid field screening of OP pesticides and nerve agents. (C) 2002 Elsevier Science B.V. All rights reserved.