This title appears in the Scientific Report :
2003
Please use the identifier:
http://dx.doi.org/10.1080/1042015031000116096 in citations.
Biological effects induced by swift heavy ions of Lithium on aqueous solution of plasmid pMTa4
Biological effects induced by swift heavy ions of Lithium on aqueous solution of plasmid pMTa4
Biological samples exposed to swift heavy ions sustain damage on different components. Damage to DNA, a critical component of a living system, has considerable biological implications. In this study aqueous solution of plasmid pMTa4 was exposed to varying fluence of swift Li-7 ions and its different...
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Personal Name(s): | Humtsoe, J. O. |
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Schneeweiss, F. H. A. / Srivastava, A. / Sarma, A. / Sharan, R. N. | |
Contributing Institute: |
Institut für Medizin; IME Abteilung Sicherheit und Strahlenschutz; S |
Published in: | Radiation effects and defects in solids, 158 (2003) S. 603 - 607 |
Imprint: |
London [u.a.]
Taylor & Francis
2003
|
Physical Description: |
603 - 607 |
DOI: |
10.1080/1042015031000116096 |
Document Type: |
Journal Article |
Research Program: |
Nukleare Sicherheitsforschung Neurowissenschaften |
Series Title: |
Radiation Effects and Defects in Solids
158 |
Subject (ZB): | |
Publikationsportal JuSER |
Biological samples exposed to swift heavy ions sustain damage on different components. Damage to DNA, a critical component of a living system, has considerable biological implications. In this study aqueous solution of plasmid pMTa4 was exposed to varying fluence of swift Li-7 ions and its different topological forms were analysed by agarose gel electrophoresis to study the induced damage. To monitor radiation labile nucleotide sequence the Li-7 ions exposed plasmid was degraded by three different restriction endonucleases and also analysed by agarose gel electrophoresis. The results show that Li-7 ions predominantly induced double strand breaks in the plasmid DNA in a dose-dependent manner and affected preferentially the GC-rich motifs of the DNA. The results suggest that Li-7 ions induce premutagenic lesions at an enhanced frequency in segments of the DNA which are rich in CG content as compared to GC-poor segments. |