This title appears in the Scientific Report :
2001
Penicillin detection by means of silicon-based field-effect structures
Penicillin detection by means of silicon-based field-effect structures
Two types of semiconductor-based field-effect biosensors, i.e., a capacitive electrolyte-isolator-semiconductor (EIS) sensor and an enzyme field-effect transistor (EnFET) with different pH-sensitive transducer materials (Si3N4 and Ta2O5) and with different enzyme immobilisation methods (heterobifunc...
Saved in:
Personal Name(s): | Poghossian, A. |
---|---|
Thust, M. / Schroth, P. / Steffen, A. / Lüth, H. / Schöning, M. J. | |
Contributing Institute: |
Institut für Halbleiterschichten und Bauelemente; ISG-1 Agrosphäre; ICG-IV Institut für Bio- und Chemosensoren; ISG-2 |
Published in: | Sensors and materials, 13 (2001) S. 207 |
Imprint: |
Tokyo
MYU
2001
|
Physical Description: |
207 |
Document Type: |
Journal Article |
Research Program: |
Ionen- und Lithographietechnik Halbleiterbauelemente und Analytik Halbleiterschichtsysteme und Mesoskopische Strukturen |
Series Title: |
Sensors and Materials
13 |
Subject (ZB): | |
Publikationsportal JuSER |
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245 | |a Penicillin detection by means of silicon-based field-effect structures | ||
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520 | |a Two types of semiconductor-based field-effect biosensors, i.e., a capacitive electrolyte-isolator-semiconductor (EIS) sensor and an enzyme field-effect transistor (EnFET) with different pH-sensitive transducer materials (Si3N4 and Ta2O5) and with different enzyme immobilisation methods (heterobifunctional cross-linking and physical adsorption) are investigated for penicillin detection. Capacitive EIS sensors with diffusion barriers are suitable for the detection of different kinds of penicillin: Penicillin G, ampicillin and amoxicillin. Compared to the conventional methods of measurement in penicillin solutions, the introduction of a diffusion barrier in combination with a suitable working buffer improves the detectable amount of penicillin to about 0.05 mug. Here, only 10 mul of the analyte is needed for a single penicillin measurement. For a penicillin FET with adsorptively immobilised penicillinase, excellent working characteristics, namely, high penicillin sensitivity (120 +/- 10 mV/mM in the linear concentration range of 0.05-1 mM), low hysteresis (< 4 mV), low detection limit of 10 muM and long lifetime (more than 5 months) were achieved by choosing Ta2O5 as a pH-sensitive transducer material, and a low capacity polymix buffer. | ||
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653 | 2 | 0 | |2 Author |a penicillin |
653 | 2 | 0 | |2 Author |a capacitive EIS sensor |
653 | 2 | 0 | |2 Author |a cross-sensitivity |
653 | 2 | 0 | |2 Author |a EnFET |
653 | 2 | 0 | |2 Author |a low detection limit |
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