This title appears in the Scientific Report :
2016
Please use the identifier:
http://hdl.handle.net/2128/10163 in citations.
Please use the identifier: http://dx.doi.org/10.1371/journal.pone.0150267 in citations.
Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering
Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering
Small proteins like amyloid beta (Aβ) monomers are related to neurodegenerative disorders by aggregation to insoluble fibrils. Small angle neutron scattering (SANS) is a nondestructive method to observe the aggregation process in solution. We show that SANS is able to resolve monomers of small molec...
Saved in:
Personal Name(s): | Zhang-Haagen, Bo |
---|---|
Biehl, Ralf (Corresponding author) / Nagel-Steger, Luitgard / Radulescu, Aurel / Richter, Dieter / Willbold, Dieter (Corresponding author) | |
Contributing Institute: |
Neutronenstreuung; ICS-1 Neutronenstreuung; JCNS-1 JCNS-FRM-II; JCNS-FRM-II Strukturbiochemie; ICS-6 |
Published in: | PLoS one, 11 (2016) 2, S. e0150267 - |
Imprint: |
Lawrence, Kan.
PLoS
2016
|
PubMed ID: |
26919121 |
DOI: |
10.1371/journal.pone.0150267 |
Document Type: |
Journal Article |
Research Program: |
Physical Basis of Diseases FRM II / MLZ |
Subject (ZB): | |
Link: |
OpenAccess OpenAccess |
Publikationsportal JuSER |
Please use the identifier: http://dx.doi.org/10.1371/journal.pone.0150267 in citations.
LEADER | 07644nam a2201057 a 4500 | ||
---|---|---|---|
001 | 808413 | ||
005 | 20220930130059.0 | ||
024 | 7 | |a 10.1371/journal.pone.0150267 |2 doi | |
024 | 7 | |a 2128/10163 |2 Handle | |
024 | 7 | |a WOS:000371274400114 |2 WOS | |
024 | 7 | |a altmetric:6012865 |2 altmetric | |
024 | 7 | |a pmid:26919121 |2 pmid | |
037 | |a FZJ-2016-02236 | ||
082 | |a 500 | ||
100 | 1 | |a Zhang-Haagen, Bo |0 P:(DE-HGF)0 |b 0 | |
245 | |a Monomeric Amyloid Beta Peptide in Hexafluoroisopropanol Detected by Small Angle Neutron Scattering | ||
260 | |a Lawrence, Kan. |c 2016 |b PLoS | ||
520 | |a Small proteins like amyloid beta (Aβ) monomers are related to neurodegenerative disorders by aggregation to insoluble fibrils. Small angle neutron scattering (SANS) is a nondestructive method to observe the aggregation process in solution. We show that SANS is able to resolve monomers of small molecular weight like Aβ for aggregation studies. We examine Aβ monomers after prolonged storing in d-hexafluoroisopropanol (dHFIP) by using SANS and dynamic light scattering (DLS). We determined the radius of gyration from SANS as 1.0±0.1 nm for Aβ1–40 and 1.6±0.1 nm for Aβ1–42 in agreement with 3D NMR structures in similar solvents suggesting a solvent surface layer with 5% increased density. After initial dissolution in dHFIP Aβ aggregates sediment with a major component of pure monomers showing a hydrodynamic radius of 1.8±0.3 nm for Aβ1–40 and 3.2±0.4 nm for Aβ1–42 including a surface layer of dHFIP solvent molecules. | ||
588 | |a Dataset connected to CrossRef | ||
650 | 2 | 7 | |a Biology |0 V:(DE-MLZ)SciArea-160 |2 V:(DE-HGF) |x 0 |
650 | 1 | 7 | |a Health and Life |0 V:(DE-MLZ)GC-130-2016 |2 V:(DE-HGF) |x 1 |
650 | 1 | 7 | |a Health and Life |0 V:(DE-MLZ)GC-130-1 |2 V:(DE-HGF) |x 0 |
693 | |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz |e KWS-2: Small angle scattering diffractometer |f NL3ao |1 EXP:(DE-MLZ)FRMII-20140101 |0 EXP:(DE-MLZ)KWS2-20140101 |5 EXP:(DE-MLZ)KWS2-20140101 |6 EXP:(DE-MLZ)NL3ao-20140101 |x 0 | ||
700 | 1 | |a Biehl, Ralf |0 P:(DE-Juel1)130542 |b 1 |e Corresponding author | |
700 | 1 | |a Nagel-Steger, Luitgard |0 P:(DE-Juel1)162443 |b 2 | |
700 | 1 | |a Radulescu, Aurel |0 P:(DE-Juel1)130905 |b 3 | |
700 | 1 | |a Richter, Dieter |0 P:(DE-Juel1)130917 |b 4 | |
700 | 1 | |a Willbold, Dieter |0 P:(DE-Juel1)132029 |b 5 |e Corresponding author | |
773 | |a 10.1371/journal.pone.0150267 |g Vol. 11, no. 2, p. e0150267 - |0 PERI:(DE-600)2267670-3 |n 2 |p e0150267 - |t PLoS one |v 11 |y 2016 |x 1932-6203 | ||
856 | 4 | |y OpenAccess |u http://juser.fz-juelich.de/record/808413/files/Monomeric%20Amyloid%20Beta%20Peptide%20in%20Hexafluoroisopropanol%20Detected%20by%20Small%20Angle%20Neutron%20Scattering_2016.pdf | |
856 | 4 | |y OpenAccess |x icon |u http://juser.fz-juelich.de/record/808413/files/Monomeric%20Amyloid%20Beta%20Peptide%20in%20Hexafluoroisopropanol%20Detected%20by%20Small%20Angle%20Neutron%20Scattering_2016.gif?subformat=icon | |
856 | 4 | |y OpenAccess |x icon-1440 |u http://juser.fz-juelich.de/record/808413/files/Monomeric%20Amyloid%20Beta%20Peptide%20in%20Hexafluoroisopropanol%20Detected%20by%20Small%20Angle%20Neutron%20Scattering_2016.jpg?subformat=icon-1440 | |
856 | 4 | |y OpenAccess |x icon-180 |u http://juser.fz-juelich.de/record/808413/files/Monomeric%20Amyloid%20Beta%20Peptide%20in%20Hexafluoroisopropanol%20Detected%20by%20Small%20Angle%20Neutron%20Scattering_2016.jpg?subformat=icon-180 | |
856 | 4 | |y OpenAccess |x icon-640 |u http://juser.fz-juelich.de/record/808413/files/Monomeric%20Amyloid%20Beta%20Peptide%20in%20Hexafluoroisopropanol%20Detected%20by%20Small%20Angle%20Neutron%20Scattering_2016.jpg?subformat=icon-640 | |
856 | 4 | |y OpenAccess |x pdfa |u http://juser.fz-juelich.de/record/808413/files/Monomeric%20Amyloid%20Beta%20Peptide%20in%20Hexafluoroisopropanol%20Detected%20by%20Small%20Angle%20Neutron%20Scattering_2016.pdf?subformat=pdfa | |
909 | C | O | |o oai:juser.fz-juelich.de:808413 |p openaire |p open_access |p OpenAPC |p driver |p VDB:MLZ |p VDB |p openCost |p dnbdelivery |
910 | 1 | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 1 |6 P:(DE-Juel1)130542 | |
910 | 1 | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-Juel1)162443 | |
910 | 1 | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)130905 | |
910 | 1 | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-Juel1)130917 | |
910 | 1 | |a Forschungszentrum Jülich GmbH |0 I:(DE-588b)5008462-8 |k FZJ |b 5 |6 P:(DE-Juel1)132029 | |
913 | 1 | |a DE-HGF |9 G:(DE-HGF)POF3-6G15 |x 0 |4 G:(DE-HGF)POF |v FRM II / MLZ |1 G:(DE-HGF)POF3-6G0 |0 G:(DE-HGF)POF3-6G15 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-600 |b Forschungsbereich Materie |l Großgeräte: Materie | |
913 | 1 | |a DE-HGF |b Key Technologies |l BioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-553 |2 G:(DE-HGF)POF3-500 |v Physical Basis of Diseases |x 1 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 | |
914 | 1 | |y 2016 | |
915 | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews | ||
915 | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)1040 |2 StatID |b Zoological Record | ||
915 | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b PLOS ONE : 2014 | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0500 |2 StatID |b DOAJ | ||
915 | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection | ||
915 | |a IF < 5 |0 StatID:(DE-HGF)9900 |2 StatID | ||
915 | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline | ||
915 | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List | ||
980 | 1 | |a UNRESTRICTED | |
980 | 1 | |a FullTexts | |
980 | |a journal | ||
980 | |a VDB | ||
980 | |a UNRESTRICTED | ||
980 | |a I:(DE-Juel1)JCNS-FRM-II-20110218 | ||
980 | |a I:(DE-Juel1)JCNS-1-20110106 | ||
980 | |a I:(DE-Juel1)ICS-1-20110106 | ||
980 | |a I:(DE-Juel1)ICS-6-20110106 | ||
980 | |a APC | ||
536 | |a Physical Basis of Diseases |0 G:(DE-HGF)POF3-553 |c POF3-553 |f POF III |x 1 | ||
536 | |a FRM II / MLZ |0 G:(DE-HGF)POF3-6G15 |f POF III |x 0 |c POF3-6G15 | ||
336 | |a ARTICLE |2 BibTeX | ||
336 | |a Nanopartikel unedler Metalle (Mg0, Al0, Gd0, Sm0) |0 0 |2 EndNote | ||
336 | |a Output Types/Journal article |2 DataCite | ||
336 | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1460613343_2947 |2 PUB:(DE-HGF) | ||
336 | |a article |2 DRIVER | ||
336 | |a JOURNAL_ARTICLE |2 ORCID | ||
920 | |k Neutronenstreuung; ICS-1 |0 I:(DE-Juel1)ICS-1-20110106 |l Neutronenstreuung |x 2 | ||
920 | |k Neutronenstreuung; JCNS-1 |0 I:(DE-Juel1)JCNS-1-20110106 |l Neutronenstreuung |x 1 | ||
920 | |k JCNS-FRM-II; JCNS-FRM-II |0 I:(DE-Juel1)JCNS-FRM-II-20110218 |l JCNS-FRM-II |x 0 | ||
920 | |l yes | ||
981 | |a I:(DE-Juel1)ICS-6-20110106 | ||
981 | |a I:(DE-Juel1)ICS-1-20110106 | ||
981 | |a I:(DE-Juel1)JCNS-1-20110106 | ||
981 | |a I:(DE-Juel1)IBI-7-20200312 | ||
981 | |a I:(DE-Juel1)IBI-8-20200312 | ||
920 | |k Strukturbiochemie; ICS-6 |0 I:(DE-Juel1)ICS-6-20110106 |l Strukturbiochemie |x 3 | ||
990 | |a Zhang-Haagen, Bo |0 P:(DE-HGF)0 |b 0 | ||
991 | |a Willbold, Dieter |0 P:(DE-Juel1)132029 |b 5 |e Corresponding author | ||
991 | |a Richter, Dieter |0 P:(DE-Juel1)130917 |b 4 | ||
991 | |a Radulescu, Aurel |0 P:(DE-Juel1)130905 |b 3 | ||
991 | |a Nagel-Steger, Luitgard |0 P:(DE-Juel1)162443 |b 2 | ||
991 | |a Biehl, Ralf |0 P:(DE-Juel1)130542 |b 1 |e Corresponding author |