Table of Contents: “... Horse Aproach Using Ustilago maydis to Study Apoplastic Maize (Zea mays) Peptides in situ -- Feeding Assay to Study the Effect of Phytocytokines on Direct and Indirect Defense in Maize -- A Quick Method to Analyze Peptide-Regulated Anthocyanin Biosynthesis...”

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Table of Contents: “... and rice -- Isolation of meiocytes and cytological analyses of male meiotic chromosomes in soybean, lettuce and maize -- Genetic and phenotypic analyses of carpel development in Arabidopsis -- Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis...”

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Table of Contents: “... Microscopy Techniques -- A Simple Semi-Hydroponic System for Studying Iron Homeostasis in Maize -- Optimizing Fe Nutrition for Algal Growth....”

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Table of Contents: “... in Maize -- Isolation and Transfection of Maize Endosperm Protoplasts -- Protoplast Isolation from Undifferentiated Maize Seedling Shoot Tissue -- Protoplast Isolation and Transfection in Wheat -- Protoplast Isolation, Transformation, and Regeneration for Forage...”

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Table of Contents: “... for in vivo doubled haploid (DH) technology in maize breeding: From haploid inducer Development to Haploid Genome Doubling -- Estimation of heterografting associated DNA methylation changes in tree crops by MSAP analysis -- In vitro fertilization system using wheat...”

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Table of Contents: “... for Crop Phenotyping and Stress Detection -- Analysis of Innate Immune Responses Against Pathogenic Bacteria in Arabidopsis, Tomato and Barley -- Performing Infection Assays of Sporisorium reilianum f. sp. Zeae in Maize -- A Method to Measure the Damage Caused...”

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Table of Contents: “...Albino Plant Formation in Androgenic Cultures: An Old Problem and New Facts -- The Maize in planta Haploid Induction Lines: A Corner Stone for Doubled Haploid Technology -- The Use of Molecular Markers for Doubled Haploid Technology: From Academia...”

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Table of Contents: “... -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR...”

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Table of Contents: “... the Constituent Genomes of Brassica nigra and B. rapa in the Hybrid B. juncea -- Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization -- Cytological Techniques to Study Cytomixis in Plant Male Meiosis...”

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Table of Contents: “... in the Developing Endosperm of Maize -- RNA Isolation and Analysis of lncRNAs from Gametophytes of Maize -- Improved Method of RNA Isolation from Laser Capture Microdissection (LCM) Derived-Plant Tissues -- Medium Throughput RNA In Situ Hybridization of Serial Sections...”

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Table of Contents: “... Transcriptional Activation -- Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR-Cas9 -- Knocking Out MicroRNA Genes in Rice with CRISPR/Cas9 -- An Agrobacterium-Mediated CRISPR-Cas9 Platform for Genome Editing in Maize -- Fluorescence Marker...”

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Table of Contents: “... -- Transcription Factors: Their Role in the Regulation of Somatic Embryogenesis in Theobroma cacao L. and other Species -- MicroRNA Expression and Regulation During Maize Somatic Embryogenesis -- Elaboration of Transcriptome during the Induction of Somatic Embryogenesis...”

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Table of Contents: “...Measuring Plant Root Traits Under Controlled and Field Conditions: Step-By-Step Procedures -- Phenotyping Crop Root Crowns: General Guidance and Specific Protocols for Maize, Wheat, and Soybean -- Developmental Analysis of Arabidopsis Root Meristem...”

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Table of Contents: “... -- Section Overview: Unravelling the Complex Epigenetic Mechanisms that Regulate Gene Activity -- Technical Review: A Hitchhikers Guide to Chromosome Conformation Capture -- 3C in Maize and Arabidopsis -- Profiling Histone Modifications in Synchronised Floral...”

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Table of Contents: “... Transcription Start Site Sequencing -- Genome-Wide TSS Identification in Maize -- Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee -- Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci...”

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Table of Contents: “...Fluorescence In Situ Hybridization in Oat -- Oat Doubled Haploids Following Maize Pollination -- Wide Hybridization between Oat and Pearl Millet -- Protocol for Producing Synthetic Polyploid Oats -- Manipulation of Oat Protoplasts for Transient...”

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Table of Contents: “... Reagents for Gene Knock-Out, Targeted Insertion, and Replacement in Wheat -- Doubled Haploid Transgenic Wheat Lines by Microspore Transformation -- Doubled Haploid Laboratory Protocol for Wheat Using Wheat-Maize Wide Hybridization -- Real-Time PCR...”

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Table of Contents: “... Microscopy -- In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes -- Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins -- Live Cell Imaging of Auxin and Cytokinin Signaling in Maize Female...”

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Table of Contents: “... Fluorescent Probes -- Using Live Cell Markers in Maize to Analyze Cell Division Orientation and Timing -- Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe....”

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