Table of Contents: “... Transcription Start Site Sequencing -- Genome-Wide TSS Identification in Maize -- Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee -- Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci...”

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Table of Contents: “... -- Transcription Factors: Their Role in the Regulation of Somatic Embryogenesis in Theobroma cacao L. and other Species -- MicroRNA Expression and Regulation During Maize Somatic Embryogenesis -- Elaboration of Transcriptome during the Induction of Somatic Embryogenesis...”

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Table of Contents: “...Measuring Plant Root Traits Under Controlled and Field Conditions: Step-By-Step Procedures -- Phenotyping Crop Root Crowns: General Guidance and Specific Protocols for Maize, Wheat, and Soybean -- Developmental Analysis of Arabidopsis Root Meristem...”

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Table of Contents: “... -- Section Overview: Unravelling the Complex Epigenetic Mechanisms that Regulate Gene Activity -- Technical Review: A Hitchhikers Guide to Chromosome Conformation Capture -- 3C in Maize and Arabidopsis -- Profiling Histone Modifications in Synchronised Floral...”

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Table of Contents: “...Fluorescence In Situ Hybridization in Oat -- Oat Doubled Haploids Following Maize Pollination -- Wide Hybridization between Oat and Pearl Millet -- Protocol for Producing Synthetic Polyploid Oats -- Manipulation of Oat Protoplasts for Transient...”

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Table of Contents: “... Microscopy -- In Vivo Ploidy Determination of Arabidopsis thaliana Male and Female Gametophytes -- Staining and Clearing of Arabidopsis Reproductive Tissue for Imaging of Fluorescent Proteins -- Live Cell Imaging of Auxin and Cytokinin Signaling in Maize Female...”

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Table of Contents: “... Reagents for Gene Knock-Out, Targeted Insertion, and Replacement in Wheat -- Doubled Haploid Transgenic Wheat Lines by Microspore Transformation -- Doubled Haploid Laboratory Protocol for Wheat Using Wheat-Maize Wide Hybridization -- Real-Time PCR...”

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Table of Contents: “... Fluorescent Probes -- Using Live Cell Markers in Maize to Analyze Cell Division Orientation and Timing -- Ratiometric Fluorescence Live Imaging Analysis of Membrane Lipid Order in Arabidopsis Mitotic Cells Using a Lipid Order-Sensitive Probe....”

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Table of Contents: “....) Transformation Using Immature Embryos -- Barley (Hordeum Vulgare L.) Transformation Using Embryogenic Pollen Cultures -- Maize (Zea Mays L.) -- Maize, Tropical (Zea Mays L.). - Finger Millet [Eleusine Coracana (L.) Gaertn] -- Oat (Avena Sativa L.) -- Rice, Indica...”

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Table of Contents: “... Transformation -- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos -- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops -- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re...”

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Table of Contents: “... Markers Used to Monitor Transposon Activity -- Molecular Biology of Maize Ac/Ds Elements – an Overview -- Gene Tagging with Engineered Ds Elements in Maize -- Plant Regeneration Methods for Rapid Generation of a Large Scale of Ds Transposant Population in Rice...”

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Table of Contents: “... of Phenotypes, Gene Expression and Protein Localization During Embryonic Root Formation in Arabidopsis -- Inducible System for Lateral Roots in Arabidopsis thaliana and Maize -- Adventitious Root Induction in Arabidopsis thaliana as a Model for in vitro Root...”

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Table of Contents: “... and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus -- Labeling Meiotic Chromosomes in Maize with Fluorescence in situ Hybridization -- Examining Female Meiocytes of Maize by Confocal Microscopy -- Three...”

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