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Pre-Clinical Models [E-Book] : Techniques and Protocols /
Table of Contents: ... Oligodendrocyte Cell Line -- Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan -- Isolation and Characterisation of Different Mesenchymal Stem Cell Populations from Rat Femur...

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Suicide Gene Therapy [E-Book] : Methods and Protocols /
Table of Contents: ... Antigen Receptor-Redirected T-Cells for Cancer Immunotherapy -- Mesenchymal Stem Cell Exosome-Mediated Prodrug Gene Therapy for Cancer -- Extracellular Vesicles as Carriers of Suicide mRNA and/or Protein in Cancer Therapy -- Production of Viral Vectors...

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Cancer Driver Genes [E-Book] : Methods and Protocols /
Table of Contents: ... Rearrangement in Mouse Embryonic Stem Cell for Cancer Gene Discovery....

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Hepatitis C Virus Protocols [E-Book] /
Table of Contents: ... Relevant HCV Chimeric Molecular Clones -- Production and Purification of Cell Culture Hepatitis C Virus -- Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture -- Molecular Identification of Transmitted/Founder Hepatitis C...

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Making Science Fun - A Tribute to Our Colleague and Friend, Prof. Antonius G. Rolink (1953-2017) [E-Book] /
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Progenitor Cells [E-Book] : Methods and Protocols /
Table of Contents: ... Pancreas -- A Novel Gene Delivery Approach Using Metal Organic Frameworks in Human Islet-Derived Progenitor Cells -- Encapsulation and Transplantation of Pancreatic Progenitor Cells -- Differentiation of Urothelium from Mouse Embryonic Stem Cells in Chemically...

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In vitro neuronal networks : from culturing methods to neuro-technological applications [E-Book] /
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Fragile-X Syndrome [E-Book] : Methods and Protocols /
Table of Contents: ..., Methylation Status and AGG Interruptions in the Fragile X-Related Disorders -- One-Step Generation of Seamless Luciferase Gene Knock-In Using CRISPR/Cas9 Genome Editing in Human Pluripotent Stem Cells -- Modeling FXS with Mouse Neural Progenitors -- Using Human...

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209
Target Identification and Validation in Drug Discovery [E-Book] : Methods and Protocols /
Table of Contents: ... Validation Studies -- Expression of Human VH Single Domains as Fc-Fusions in Mammalian Cells -- The NeuroSphere Assay (NSA) Applied to Neural Stem Cells (NSCs) and Cancer Stem Cells (CSCs) -- 3D-3 Tumor Models in Drug Discovery for Analysis of Immune Cell...

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CRISPR Gene Editing [E-Book] : Methods and Protocols /
Table of Contents: ... (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors -- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs -- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9...

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Developmental Toxicology [E-Book] : Methods and Protocols /
Table of Contents: ...In Vitro Models in Developmental Toxicology -- Use of Primary Mouse Embryonic Fibroblasts in Developmental Toxicity Assessments -- Induced Pluripotent Stem Cells (iPSCs) in Developmental Toxicology -- Neural Crest Cell Models of Development and Toxicity...

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Chimera Research [E-Book] : Methods and Protocols /
Table of Contents: ...Frontiers of Pluripotency -- Highly Efficient Derivation of Pluripotent Stem Cells from Mouse Preimplantation and Postimplantation Embryos in Serum-Free Conditions -- Derivation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells...

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Stem Cell Mobilization [E-Book] : Methods and Protocols /
Table of Contents: ...Evolution of Peripheral Blood Stem Cell Transplantation -- In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents -- Colony Formation: An Assay of Hematopoietic Progenitor Cells -- Mobilization and Collection of Peripheral Blood Stem Cells...

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214
Nanotechnology Characterization Tools for Tissue Engineering and Medical Therapy [E-Book] /
Table of Contents: ...Nanotechnology-Based Stem Cell Tissue Engineering with a Focus on Regeneration of Cardiovascular Systems -- Characterization Tools for Mechanical Probing of Biomimetic Materials -- Open-Channel Separation Techniques for the Characterization...

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Immunological Tolerance [E-Book] : Methods and Protocols /
Table of Contents: ...Isolation and Characterisation of Human Adipose-derived Stem Cells -- Isolation of Murine Hematopoietic Stem Cells -- Generation of Antigen-specific T cells from Human Induced Pluripotent Stem Cells -- Isolation of Human Regulatory T Lymphocytes...

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Neural Stem Cells [E-Book] : Methods and Protocols /
Table of Contents: ...Generation of Neural Stem Cells from Induced Pluripotent Stem Cells -- Making NSC and Neurons from Patient Derived Tissue Samples -- Generation of definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord...

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Skin Stem Cells [E-Book] : Methods and Protocols /
Table of Contents: ...In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging -- Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells -- Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human...

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218
Bone Research Protocols [E-Book] /
Table of Contents: ...Isolation and Generation of Osteoclasts -- Isolation and Generation of Osteoblasts -- Isolation, Purification, Generation, and Culture of Osteocytes -- Isolation, Differentiation, and Characterisation of Human Bone Marrow Stem Cells In Vitro and In Vivo...

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Kidney Organogenesis [E-Book] : Methods and Protocols /
Table of Contents: ... -- Dissociation of Tissues for Single Cell Analysis -- Long Term Culture of Nephron Progenitor Cells Ex Vivo -- 3D Cell Culture Models of Epithelial Tissues -- Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells -- Generating Multiple...

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Mouse Cell Culture [E-Book] : Methods and Protocols /
Table of Contents: ...The Applicability of Mouse Models to the Study of Human Disease -- Optimizing the Cell Culture Microenvironment -- Propagation and Maintenance of Mouse Embryonic Stem Cells -- Production of High Titer Lentiviral Particles for Stable Genetic Modification...

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