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Extracellular vesicles : applications to regenerative medicine, therapeutics and diagnostics [E-Book] /
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Neural Progenitor Cells [E-Book] : Methods and Protocols /
Table of Contents: ...-Floating Neurospheres -- Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord -- Culturing and Expansion of "Clinical Grade" Neural Stem Cells from the Fetal Human Central Nervous System -- Microglia Isolation from Neural Stem Cell-Enriched...

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Hedgehog Signaling [E-Book] : Methods and Protocols  /
Table of Contents: ...Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells -- Evaluating Hedgehog Acyltransferase Activity and Inhibition Using the Acylation-Coupled Lipophilic Induction of Polarization (Acyl-cLIP) Assay -- Analysis...

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Glycosaminoglycans [E-Book] : Methods and Protocols /
Table of Contents: ... Strategies -- Xyloside Derivatives as Molecular Tools to Selectively Inhibit Heparan Sulfate and Chondroitin Sulfate Proteoglycan Biosynthesis -- A Strategic Approach to Identification of Selective Inhibitors of Cancer Stem Cells -- Applications of Xylosides...

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Vascular Tissue Engineering [E-Book] : Methods and Protocols /
Table of Contents: ...Differentiating Human Pluripotent Stem Cells to Vascular Endothelial Cells for Regenerative Medicine, Tissue Engineering, and Disease Modeling -- Generating Monocyte Derived Endothelial-Like Cells for Vascular Regeneration -- Methods for Differentiating...

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Embryonic Stem Cell Protocols [E-Book] /
Table of Contents: ...Dissecting Molecular Phenotypes through FACS-Based Pooled CRISPR Screens -- Feeder Free Human Embryonic Stem Cell Culture under Defined Culture Conditions -- Rat Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish -- Transient...

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Tissue Scaffolds [E-Book] /
Table of Contents: ... submucosa for clinical applications in corneal surface reconstruction -- Tissue scaffolds derived from fish swim bladder -- Bone marrow derived mesenchymal stem cells from different species of animals -- Proteomics protocols for characterization of xeno...

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Material-based mechanobiology [E-Book] /
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Medulloblastoma [E-Book] : Methods and Protocols  /
Table of Contents: ... approaches to obtain medulloblastoma stem cells from bulk cultures -- Drug Screening assays on Medulloblastoma Stem Cells using compound libraries -- 3D-on-2D: A physiologically-relevant and gel-free in vitro co-culture method to assay anti-metastatic agents...

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HIV Reservoirs [E-Book] : Methods and Protocols /
Table of Contents: ..., and Approaches to a Cure -- Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research -- Inducing long-term HIV-1 latency in the TKO-BLT mouse model -- In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within...

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Measuring Cerebellar Function [E-Book] /
Table of Contents: ...Cerebellar Modeling using Human Induced Pluripotent Stem Cells -- From Cerebellar Genes to Behaviors in Zebrafish -- Silencing the Output of Cerebellar Neurons using Cell Type-Specific Genetic Deletion of Vesicular GABA and Glutamate Transporters...

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72
Innovative Bioceramics in Translational Medicine. I. Fundamental Research [E-Book] /
Table of Contents: ... Materials for Medical and Engineering Applications -- Stem Cells and Proteomics in Biomaterials and Biomedical Applications -- Antimicrobial Bioceramics for Biomedical Applications -- Surface Functionalization of Titanium for the Control and Treatment...

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Extracellular Vesicles in Diagnosis and Therapy [E-Book] /
Table of Contents: ... Placental Extracellular Vesicles -- Isolation and Characterization of Extracellular Vesicles Secreted from Human Pluripotent Stem Cell-Derived Cardiovascular Progenitor Cells -- Isolation and Characterization of Salivary Exosomes for Cancer Biomarker Discovery...

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Epiblast Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...Establishment of Mouse Epiblast Stem Cells -- L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell -- Generation of Epiblast-Like Cells -- Identification...

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Stem Cell Assays [E-Book] : Methods and Protocols  /
Table of Contents: ...Rapid and Highly Efficient Isolation and Purification of Human Induced Pluripotent Stem Cells -- Artificial Activation of Murine Oocytes using Strontium to Derive Haploid and Diploid Parthenotes -- Generation of Human iPSC from Small Volume Peripheral...

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Circadian Regulation : Methods and Protocols [E-Book] /
Table of Contents: ... locomotor activity in home cages -- Recording of diurnal gene expression in peripheral organs of mice using the RT-Biolumicorder -- Isolation and sorting of epidermal interfollicular stem cells for the study of circadian rhythms -- Detecting circadian rhythms...

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Contemporary Approaches to the Study of Pain : From Molecules to Neural Networks [E-Book] /
Table of Contents: ... Preparation -- Electrophysiological Recording Techniques from Human Dorsal Root Ganglion -- Human Pluripotent Stem Cell-Derived Sensory Neurons: A New Translational Approach to Study Mechanisms of Sensitization? -- Intraspinal Transplantation of Precursors...

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Cardiac Tissue Engineering : Methods and Protocols [E-Book] /
Table of Contents: ...CRISPR Library Screening in Cultured Cardiomyocytes -- Protein and mRNA Quantification in Small Samples of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates -- Self-Assembled Heterotypic Cardiac Spheroids from Human...

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Applications of Genome Modulation and Editing [E-Book] /
Liu, Jun
2022
Table of Contents: ... pluripotent stem cells -- Microinjection of zygotes for CRISPR/Cas9 mediated insertion of transgenes into the murine Rosa26 safe harbour -- CRISPR-on for endogenous activation of SMARCA4 expression in bovine embryos -- CRISPR/Cas mutagenesis to generate novel...

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Induced Pluripotent Stem (iPS) Cells [E-Book] : Methods and Protocols  /