This title appears in the Scientific Report :
2008
Please use the identifier:
http://dx.doi.org/10.1016/j.jneumeth.2007.10.011 in citations.
Challenge by the murine brain: Multi-pinhole SPECT of 123I-labelled pharmaceuticals
Challenge by the murine brain: Multi-pinhole SPECT of 123I-labelled pharmaceuticals
This protocol presents an improved method for SPECT imaging based on multi-pinhole techniques, applied to the visualisation of neurotracers in small animal models. Three types of collimators with 6-pinhole apertures adapted to special requirements for the imaging of the brain of mice and rats and to...
Saved in:
Personal Name(s): | Pissarek, M.B. |
---|---|
Oros-Peusquens, A.-M. / Schramm, N.U. | |
Contributing Institute: |
Institut für Neurowissenschaften und Biophysik - Medizin; INB-3 Zentralinstitut für Elektronik; ZEL Nuklearchemie; INB-4 |
Published in: | Journal of neuroscience methods, 168 (2008) S. 282 - 292 |
Imprint: |
Amsterdam [u.a.]
Elsevier Science
2008
|
Physical Description: |
282 - 292 |
DOI: |
10.1016/j.jneumeth.2007.10.011 |
PubMed ID: |
18061274 |
Document Type: |
Journal Article |
Research Program: |
Funktion und Dysfunktion des Nervensystems |
Series Title: |
Journal of Neuroscience Methods
168 |
Subject (ZB): | |
Publikationsportal JuSER |
This protocol presents an improved method for SPECT imaging based on multi-pinhole techniques, applied to the visualisation of neurotracers in small animal models. Three types of collimators with 6-pinhole apertures adapted to special requirements for the imaging of the brain of mice and rats and to full body imaging in mice are employed in the experiments. A conventional triple-headed TRIAD/Trionix SPECT system was upgraded with pyramidal supports and shieldings onto the multi-pinhole collimators were installed. The system was employed for the assessment of the uptake of [123I]FP-CIT and [123I]IBZM, well known tracers of dopamine transport and dopamine D2/D3 receptors, respectively. Requirements regarding the applied radioactivity are reported, as well as further conditions determining the effectiveness of the detection of the uptake of [123I]FP-CIT and [123I]IBZM. The measurements in mice required only 20-25% of the activity described in previous studies. Dynamic measurements are presented, with a time resolution as high as 10 min in the brain of rats. Due to the lower signal intensity obtained for mice, the time resolution was 42min for [123I]FP-CIT, with a ratio ROI/background of 5.4, and 17 min for [123I]IBZM, with the ratio ROI/background of 4.5 (1.6-7.4). |