Table of Contents: “... in Botrytis cinerea -- Exopolysaccharide Production and Precipitation Method as a Tool to Study Virulence Factors -- Virulence-Related Assays for Investigation of the Acidovorax citrulli-Cucurbitaceae Pathosystem -- Dual-Fluorescence Chromosome-Located Labeling...”

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Table of Contents: “... Assembly Using ReIN-Map -- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly -- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE -- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways...”

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Table of Contents: “...-Derived Chlamydia Using Laser Microdissection -- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions -- Mutagenesis of Chlamydia trachomatis Using TargeTron -- Forward and Reverse Genetic Analysis of Chlamydia -- A Co-Infection Model...”

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Table of Contents: “...Pathway and Gene Discovery from Natural Hosts and Organisms -- Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity -- Antisense RNA Elements for Downregulating Expression -- Chemically Inducible Chromosomal Evolution (CIChE...”

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Table of Contents: “... Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes -- Use of RP4::mini-Mu for Gene Transfer -- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu....”

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Table of Contents: “... and Their Effects on Biofilm Formation and Gene Expression -- Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth -- Detection of Bacterial Quorum Sensing Molecules -- Generating Chromosome...”

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Table of Contents: “... of Escherichia coli Transcription Factors -- Modular assembly of Synthetic Secondary Chromosomes -- High-resolution Characterization of DNA/Protein Complexes in Living Bacteria -- Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor...”

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Subject (ZB): “...chromosome...”

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Table of Contents: “... -- Generation and Analysis of Chromosomal Contact Maps of Yeast Species -- A Versatile Procedure to Generate Genome-Wide Spatio-Temporal Program of Replication in Yeast Species -- Single-Step Affinity Purification (ssAP) and Mass Spectrometry of Macromolecular...”

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Table of Contents: “... -- Engineering Infectious cDNAs of Coronavirus as Bacterial Artificial Chromosomes -- Protein Histochemistry Using Coronaviral Spike Proteins: Studying Binding Profiles and Sialic Acid Requirements for Attachment to Tissues -- Identification of Protein Receptors...”

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Table of Contents: “... Assessment of Genetic Buffering by Kinetic Analysis of Cell Arrays -- Detection of Short-Range Chromatin Interactions by Chromosome Conformation Capture (3C) in Yeast -- Chromosome Conformation Capture (3C) of Tandem Arrays in Yeast -- Global Analysis...”

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Table of Contents: “... -- Multilocus Sequence Typing (MLST) of Staphylococcus aureus -- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA -- Genetic Interruption of Target Genes for Investigation of Virulence Factors -- Molecular Analysis of Staphylococcal Superantigens...”

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Table of Contents: “... Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology -- Construction, Production, and Purification of Recombinant Adenovirus Vectors -- Scalable Production of Adenovirus Vectors -- Canine Adenovirus Downstream Processing Protocol -- Production...”

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Table of Contents: “... by RNA Sequencing -- Gene Amplification and qRT-PCR -- The Standard European Vector architecture (SEVA) plasmid toolkit -- Chromosomal integration of transcriptional fusions -- A Method to Capture Large DNA Fragments from Genomic DNA -- Transposon Mutagenesis...”

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Table of Contents: “... bioluminescence -- Microcolony method -- Direct epiflourescence filter technique (DEFT) -- Chemscan -- Protein fingerprinting by polyacrylamide gel electrophoresis -- Karyotyping (chromosome fingerprinting) -- Immunoanalysis -- Hybridization using DNA probes...”

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Table of Contents: “... and Theory -- 2 Fields of Application -- 3 Practical Recommendations -- References -- 4 Schwanniomyces occidentalis -- 1 History of Schwanniomyces occidentalis Research -- 2 Physiology -- 3 Media -- 4 Available Strains -- 5 Genetic Techniques -- 6 Chromosomal DNA...”

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