Table of Contents: “...: Introduction to stem cell biology -- Chapter 26: Transcriptomics -- Chapter 27: Development of Biomarker -- Chapter 28: Single cell genomics -- Chapter 29: Big data Analysis....”

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Table of Contents: “... Homologous Recombination in Mouse Embryonic Stem Cells -- Branch Migration Activity of Rad54 Protein -- Holliday Junction Resolution -- Identification and Analysis of Different Types of UFBs -- Intrachromosomal Recombination in Yeast -- Genome-Wide Analysis...”

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Table of Contents: “... in the Mouse via Embryonic Stem Cells -- Blastocyst Microinjection with Embryonic Stem Cells -- Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos -- Embryo Transfer Surgery -- Non-Surgical Embryo Transfer Protocol for use...”

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Table of Contents: “... -- Advances in Vaccines -- Human Pluripotent Stem Cells: Applications and Challenges for Regenerative Medicine and Disease Modeling -- Addressing the Manufacturing Challenges of Cell-Based Therapies -- Bioprinting Technologies in Tissue Engineering -- Gene Therapy....”

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Table of Contents: “...Frontiers of Pluripotency -- Highly Efficient Derivation of Pluripotent Stem Cells from Mouse Preimplantation and Postimplantation Embryos in Serum-Free Conditions -- Derivation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells...”

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Table of Contents: “... Rearrangement in Mouse Embryonic Stem Cell for Cancer Gene Discovery....”

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Table of Contents: “... (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors -- Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs -- Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9...”

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Table of Contents: “..., Methylation Status and AGG Interruptions in the Fragile X-Related Disorders -- One-Step Generation of Seamless Luciferase Gene Knock-In Using CRISPR/Cas9 Genome Editing in Human Pluripotent Stem Cells -- Modeling FXS with Mouse Neural Progenitors -- Using Human...”

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Table of Contents: “...Identification of Disease Susceptibility Alleles in the Next Generation Sequencing Era -- Induced Pluripotent Stem Cells in Disease Modeling and Gene Identification -- Development of Targeted Therapies Based on Gene Modification -- What can we Learn...”

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Table of Contents: “... MicroRNAs from Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration.-Quantitative Analysis of Precursors MicroRNAs and their Respective Mature MicroRNAs in Cancer Exosomes Overtime.-Quantum Language of MicroRNA: Application for New...”

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Table of Contents: “... -- Non-Canonical ATM Activation and Signaling in Response to Transcription-Blocking DNA Damage -- Study of ATM Phosphorylation by Cdk5 in Neuronal Cells -- DNA Damage Response in Human Stem Cells and Neural Descendants -- A Patient-Specific Stem Cell Model...”

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Table of Contents: “... Recognition from deepCAGE data -- Bioinformatics Pipeline for Transcriptome Sequencing Analysis -- CRISPR/Cas-9 Genome Editing in Embryonic Stem Cells -- Targeted Gene Activation Using RNA-Guided Nucleases....”

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Table of Contents: “...Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells -- Mutagenesis and Genome Engineering of Epstein-Barr Virus in Cultured Human Cells by CRISPR/Cas9 -- Use of CRISPR/Cas Genome Editing...”

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Table of Contents: “... Topology using Chromosome Conformation Capture Coupled to High-throughput Sequencing (3C-Seq) -- Using an Inducible CRISPR-Cas9-KRAB Effector System to Dissect Transcriptional Regulation in Human Embryonic Stem Cells -- In vitro assay to Study Histone...”

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Table of Contents: “... and Polyphenols -- MicroRNA Profiling of Exosomes -- MiRNA Profiling in Human Induced Pluripotent Stem Cells -- MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells -- TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery...”

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Table of Contents: “... Vectors In Mildly Acidic Ph Conditions -- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G -- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral...”

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Table of Contents: “... Of Xenomitochondrial Embryonic Stem Cells For The Production Of Live Xenomitochondrial Mice -- Import Of Fluorescent RNA Into Mitochondria Of Living Cells -- Purification And Comparative Assay Of The Human Mitochondrial Replicative DNA Helicase -- Expression...”

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Table of Contents: “... Using Human Mesenchymal Stem Cells to Deliver Growth Factors in the Skeletal Muscle of a Familial ALS Rat Model.- Gene Therapy Models of Alzheimer’s Disease and Other Dementias.- Viral Vector-Based Modeling of Neurodegenerative Disorders: Parkinson’s Disease...”

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Table of Contents: “...: Evaluation of Alu Activity and Recovery of de novo Inserts -- The Engineered SVA Trans-Mobilization Assay -- Detection of LINE-1 RNAs by Northern Blot -- Monitoring Long Interspersed Nuclear Element 1 Expression during Mouse Embryonic Stem Cells Differentiation...”

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