1
Synthetic Biology [E-Book] : Methods and Protocols /
Table of Contents: ... of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems -- Production of a Human Cell Line with a Plant Chromosome -- Bacterial Genome Editing with CRISPR-Cas9: Taking Clostridium beijerinckii as an Example -- CRISPR-Cas9-Mediated Genome Editing...

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Plant Chromatin Dynamics [E-Book] : Methods and Protocols /
Table of Contents: ... -- Section Overview: Unravelling the Complex Epigenetic Mechanisms that Regulate Gene Activity -- Technical Review: A Hitchhikers Guide to Chromosome Conformation Capture -- 3C in Maize and Arabidopsis -- Profiling Histone Modifications in Synchronised Floral...

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Bacteriophages [E-Book] : Methods and Protocols, Volume 3 /
Table of Contents: ... Reaction and Genome Manipulation via Electroporation of DNA Transposition Complexes -- Use of RP4::mini-Mu for Gene Transfer -- Muprints and Whole Genome Insertion Scans: Methods for Investigating Chromosome Accessibility and DNA Dynamics using Bacteriophage Mu....

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Erythropoiesis [E-Book] : Methods and Protocols /
Table of Contents: ... of Erythroblast Enucleation -- High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure -- Chromatin Immunoprecipitation (ChIP) with Erythroid Samples -- Chromosome Conformation Capture (3C and Higher) with Erythroid Samples -- Genome Editing...

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Bacterial Chromatin [E-Book] : Methods and Protocols /
Table of Contents: ... of Escherichia coli Transcription Factors -- Modular assembly of Synthetic Secondary Chromosomes -- High-resolution Characterization of DNA/Protein Complexes in Living Bacteria -- Imaging of Transcription and Replication in the Bacterial Chromosome with Multicolor...

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Mouse Oocyte Development [E-Book] : Methods and Protocols /
Table of Contents: ... -- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin -- Photoactivation of Actin in Mouse Oocyte -- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes -- Micropipette Aspiration of Oocytes to Assess Cortical Tension....

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Cellular Heterogeneity [E-Book] : Methods and Protocols /
Table of Contents: ... Structural Chromosomal Aberrations -- FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA-Sequencing -- Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy....

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Molecular Motors [E-Book] : Methods and Protocols /
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Host-Pathogen Interactions [E-Book] : Methods and Protocols /
Table of Contents: ... and Their Effects on Biofilm Formation and Gene Expression -- Serial Dilution-Based Growth Curves and Growth Curve Synchronization for High-Resolution Time Series of Bacterial Biofilm Growth -- Detection of Bacterial Quorum Sensing Molecules -- Generating Chromosome...

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Histone Variants [E-Book] : Methods and Protocols /
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Cell Cycle Synchronization [E-Book] : Methods and Protocols /
Table of Contents: ... of Cells by Inhibitors of DNA Replication: Analysis by Flow Cytometry -- Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias Latipes -- Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyrate -- Chemically...

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Histones [E-Book] : Methods and Protocols /
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Fluorescence In Situ Hybridization (FISH) [E-Book] : Application Guide /
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Plant Genomics [E-Book] : Methods and Protocols /
Table of Contents: ... Lines for QTL Mapping -- Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing -- Circular Chromosome Conformation Capture in Plants -- Genome Wide Profiling of Histone Modifications and Histone Variants...

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The Mitotic Exit Network [E-Book] : Methods and Protocols /
Table of Contents: ... of the Mitotic Exit Network at Spindle Pole Bodies -- Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays -- Analysis of the Functionality of the Mitotic Checkpoints -- Cdc14 and Chromosome Condensation...

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HDAC/HAT Function Assessment and Inhibitor Development [E-Book] : Methods and Protocols /
Table of Contents: ...Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells -- Assessment of HDACi-Induced Protein Cleavage by Caspases -- Assessment of HDACi-Induced Cytotoxicity -- Analysis of HDACi-Induced Changes in Chromosomal Passenger...

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Cohesin and Condensin [E-Book] : Methods and Protocols /
Table of Contents: ...A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts -- An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex -- Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl -- Detection...

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Telomeres and Telomerase [E-Book] : Methods and Protocols /
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Plant Germline Development [E-Book] : Methods and Protocols /
Table of Contents: ... Spreading and Image Quality for the Study of Chromosomes in Plant Tissues -- Whole Mount RNA-FISH on Ovules and Developing Seeds -- Analysis of Peroxisome Biogenesis in Pollen by Confocal Microscopy and Transmission Electron Microscopy -- Transmission Electron...

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The Bacterial Nucleoid [E-Book] : Methods and Protocols /