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Oral Biology [E-Book] : Molecular Techniques and Applications /
Table of Contents: ... -- Culturing Adipose-Derived Stem Cells Under Serum-Free Conditions -- Quantitative Real-Time Gene Profiling of Human Alveolar Osteoblasts Using a One-Step System -- Fabrication and Characterization of Decellularized Periodontal Ligament Cell Sheet Constructs...

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Golgi [E-Book] : Methods and Protocols /
Table of Contents: ... in Cell Polarity and Morphogenesis in Three-Dimensional Human Pluripotent Stem Cell Cysts -- Immunofluorescence Microscopy of the Mammalian Golgi Apparatus -- Studying the Organization of the Golgi by Super-Resolution Microscopy -- Super-Resolution Live Imaging...

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Signal Transduction Immunohistochemistry [E-Book] : Methods and Protocols /
Table of Contents: ... -- Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells -- Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments -- Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags...

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Poly(ADP-Ribose) Polymerase [E-Book] : Methods and Protocols /
Table of Contents: ... that Affect PARP1 Activity In Vivo -- Generating PARP Knockout D. Melanogaster with CRISPR/Cas9 System -- TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice -- Cell-Based Screening for New PARP Inhibitors Utilizing PARG Mutated Mouse Embryonic Stem Cells...

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Brain Organoid Research [E-Book] /
Table of Contents: ... -- Generation of Three-Dimensional Retinal Tissue with Physiologically Competent, Light Sensitive Photoreceptors from Human Induced Pluripotent Stem Cells -- Quantitative Assessment of Fluorescent Reporter Expression in 3D Retinal Organoids -- A Three-Dimensional...

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Histochemistry of Single Molecules [E-Book] : Methods and Protocols  /
Table of Contents: ... using Fluorescent Probes -- Simultaneous Labelling of Adipogenic and Osteogenic Differentiating Stem Cells for Live Confocal Analysis -- Lectin Histochemistry: Historical Perspectives, State of The Art and Future Directions -- Histochemical...

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Gene, Drug, and Tissue Engineering [E-Book] /
Table of Contents: ... for Enhancing Compound Production -- A Review of Stem Cell Technology Targeting Hepatocyte Growth as an Alternative to Organ Transplantation -- Novel Nanotechnology-Driven Prototypes for AI-Enriched Implanted Prosthetics Following Organ Failure -- Simplified CRISPR...

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CCN Proteins [E-Book] : Methods and Protocols  /
Table of Contents: ... Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells -- Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo -- Angiogenesis Assays for the Analysis of CCN Proteins -- Using...

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Alzheimer's Disease [E-Book] : Methods and Protocols /
Table of Contents: ... Alterations in Alzheimer's Disease -- Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neurons and Glia for the Elucidation of Pathogenic Mechanisms in Alzheimer's Disease -- Modeling Alzheimer's Disease using Human Brain Organoids -- 3D Biohybrid StarPEG...

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Muscular Dystrophy Therapeutics [E-Book] : Methods and Protocols /
Table of Contents: ... Expression with Duchenne Muscular Dystrophy Exon 45 Skipping in Induced-Pluripotent Stem Cell-Derived Cardiomyocytes -- Quantitative Evaluation of Exon Skipping in Urine-Derived Cells for Duchenne Muscular Dystrophy -- Use of Glycine to Augment Exon Skipping...

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Tumor Angiogenesis Assays [E-Book] : Methods and Protocols  /
Table of Contents: ... to study vascular co-option -- Development of Experimental Three-Dimensional Tumor Models to Study Glioblastoma Cancer Stem Cells and Tumor Microenvironment -- IKOSA® CAM Assay application to quantify blood vessels on chick chorioallantoic membrane (CAM...

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Hematopoietic Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...The Factory of Blood Production: Hematopoietic Stem Cells -- Detection of DNA Damage in Hematopoietic Stem Cells -- Evaluating Histone Acetylation in Mouse Hematopoietic Stem and Progenitor Cells Using Chromatin Immunoprecipitation -- CRISPR Gene Editing...

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Transposable Elements [E-Book] : Methods and Protocols /
Table of Contents: ... Macromolecules -- LINE-1 retrotransposition assays in embryonic stem cells -- Detecting somatic transposable element insertions in Drosophila tissues -- Precise and scarless insertion of transposable elements by Cas9-mediated genome engineering -- Epigenetic editing...

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Retinitis Pigmentosa [E-Book] /
Table of Contents: ... and preclinical testing -- CRISPR manipulations in stem cell lines -- CRISPR-mediated genome engineering in cell lines -- CRISPR off-targeting -- CRISPR manipulations in stem cell lines -- Generation of Human iPSC-Derived Retinal Organoids for Assessment of AAV...

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Retinal Ganglion Cells [E-Book] : Methods and Protocols /
Mead, Ben
2023
Table of Contents: ... Gun DiOlistic Labeling of Retinal Ganglion Cells -- Differentiation of Human Embryonic/Induced-Pluripotent Stem Cells to Retinal Ganglion Cells -- Laser-Induced Ocular Hypertension in a Mouse Model of Glaucoma -- Silicone Oil-Induced Ocular Hypertension...

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Calcium Phosphate Nanocoatings for Bone Regeneration [E-Book] /
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Nanooptics and Photonics, Nanochemistry and Nanobiotechnology, and Their Applications [E-Book] : Selected Proceedings of the IX International Conference Nanotechnology and Nanomaterials (NANO2021), 25-28 August 2021, Lviv, Ukraine /
Table of Contents: ... use of nanocomplexes.-Extraction and properties of nanocellulose from hemp fibers -- Impact of gold nanoparticles on metabolic and antioxidant status of cryopreserved mesenchymal stem cells from adipose tissue -- Chemical transformations of phosphoric acid...

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Hepatic Stellate Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update -- Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells -- Testing Cell Migration, Invasion, Proliferation, and Apoptosis in Hepatic Stellate Cells...

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Spermatogonial Stem Cells [E-Book] : Methods and Protocols  /
Table of Contents: ...Introduction: The Why's and How's for Studying Spermatogenesis and Spermatogonial Stem Cells -- Optimizing Testicular Fixation-Embedding Procedures for an Improved Morphofunctional Evaluation of Mammalian Spermatogonia -- Single-Molecule Fluorescence...

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Stem Cell-Based Neural Model Systems for Brain Disorders [E-Book] /
Table of Contents: ...Generation of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture -- Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells -- Generation and Co-Culture of Cortical Glutamatergic...

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