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PCR Primer Design [E-Book] /
Table of Contents: ... -- Identification of gene copy number in the transgenic plants by quantitative polymerase chain reaction (qPCR) -- qPrimerDB: A powerful and user-friendly database for qPCR primer design -- PCR primer design for the rapidly evolving SARS-CoV-2 genome -- Universal primers...

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Protocols in Advanced Genomics and Allied Techniques [E-Book] /
Pal, Aruna
2022
Table of Contents: ...Chapter 1: Conventional and Basic genomic techniques -- Chapter 2: Recombinant DNA technology -- Chapter 3: Mitochondrial Genetics -- Chapter 4: Basic principle of Cloning -- Chapter 5: Immunohistochemistry -- Chapter 6: Western blotting -- Chapter 7...

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Pseudogenes [E-Book] : Functions and Protocols /
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Ribozymes [E-Book] : Methods and Protocols  /
Table of Contents: ...Conjugation as a Highly Sensitive Assay to Study Group II Intron Splicing in vivo -- Co-transcriptional Analysis of Self-cleaving Ribozymes and their Ligand Dependence -- Cloning and Detection of Genomic Retrozymes and their circRNA Intermediates...

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Epistasis [E-Book] : Methods and Protocols  /
Table of Contents: ... -- Simulating Evolution in Asexual Populations with Epistasis -- Protocol for Construction of Genome-Wide Epistatic SNP Networks using WISH-R Package -- Brief survey on Machine Learning in Epistasis -- First-Order Correction of Statistical Significance for Screening...

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Yarrowia lipolytica [E-Book] : Methods and Protocols  /
Table of Contents: ...Genome Engineering of Yarrowia lipolytica with the piggyBac Transposon System -- Genome Editing in Y. lipolytica Using TALENs -- EasyCloneYALI: Toolbox for CRISPR-mediated Integrations and Deletions in Yarrowia lipolytica -- Simultaneous gene excision...

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TET Proteins and DNA Demethylation [E-Book] : Methods and Protocols  /
Table of Contents: ...Reduced Bisulfite-Sequencing: Quantitative Base-Resolution Sequencing of 5-Formylcytosine -- Aba-Seq: High-Resolution Enzymatic Mapping of Genomic 5-Hydroxymethylcytosine -- Estimating Global Methylation and Erasure Using Low-Coverage Whole Genome...

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Enhancers and Promoters [E-Book] : Methods and Protocols  /
Table of Contents: ... at Selected Genomic Loci And Alleles -- Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes -- Low Input Targeted Chromatin Capture (low-T2C) -- Proximity Ligation-assisted ChIP-Seq (PLAC-Seq) -- Analysis of Enhancer-promoter Interactions...

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Homologous Recombination [E-Book] : Methods and Protocols  /
Table of Contents: ...Detection of DNA Double Strand Breaks by -H2AX Immunodetection -- END-seq: An Unbiased, High-Resolution and Genome-Wide Approach to Map DNA Double Strand Breaks and Resection in Human Cells -- Resection of a DNA Double-Strand Break by Alkaline Gel...

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Trypanosomatids [E-Book] : Methods and Protocols  /
Table of Contents: ... Stages -- Genome-Wide Proteomics and Phosphoproteomics Analysis of Trypanosoma cruzi during Differentiation -- Genome-Wide Proteomics and Phosphoproteomics Analysis of Leishmania spp. during Differentiation -- CRISPR/Cas9 Technology Applied to the Study...

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Molecular Toxicology Protocols [E-Book] /
Table of Contents: ... with Bioinformatics Approaches to Investigate Regulatory Landscape of Transcription Modulators in Breast Cancer Cells -- Analysis of RNA Sequencing Data using CLC Genomics Workbench -- Using Bronchoalveolar Lavage to Evaluate Changes in Pulmonary Diseases -- Fast...

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DNA Electrophoresis [E-Book] : Methods and Protocols  /
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RNA Chaperones [E-Book] : Methods and Protocols  /
Table of Contents: ... by Chemical Probing with DMS and CMCT -- Disordered RNA Binding Region Prediction with DisoRDPbind -- Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci -- Site-Specific Dual-Color...

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Genotype Phenotype Coupling [E-Book] : Methods and Protocols  /
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RNA-Chromatin Interactions [E-Book] : Methods and Protocols  /
Table of Contents: ... Transfer Difference NMR for Determination of Protein Binding Sites on RNA Guanine Quadruplexes -- Mapping Transcriptome-wide and Genome-wide RNA-DNA Contacts with Chromatin-Associated RNA Sequencing (ChAR-seq) -- Identification of cis-elements for RNA...

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RNA Spectroscopy [E-Book] : Methods and Protocols  /
Table of Contents: ... Studies -- Analysis of the HIV-1 Genomic RNA Dimerization Initiation Site Binding to Aminoglycoside Antibiotics using Isothermal Titration -- Use of Fluorescent Nucleotides to Map RNA-Binding Sites on Protein Surface -- Fluorescent Oligonucleotide Probes...

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Radiation Cytogenetics [E-Book] : Methods and Protocols /
Table of Contents: ... Chromosomes -- PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations -- Modified PNA Telomere and Centromere FISH Protocols -- Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal...

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Adeno-Associated Virus Vectors [E-Book] : Design and Delivery /
Table of Contents: ...1. Design of AAV vectors for Delivery of RNAi -- 2. Design of AAV Vectors for Delivery of Large or Multiple Transgenes -- 3. Ligand Coupling to the AAV Capsid for Cell-Specific Gene Transfer -- 4. Quantitative and Digital Droplet-based AAV Genome...

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Fragile-X Syndrome [E-Book] : Methods and Protocols /
Table of Contents: ..., Methylation Status and AGG Interruptions in the Fragile X-Related Disorders -- One-Step Generation of Seamless Luciferase Gene Knock-In Using CRISPR/Cas9 Genome Editing in Human Pluripotent Stem Cells -- Modeling FXS with Mouse Neural Progenitors -- Using Human...

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Epitranscriptomics [E-Book] : Methods and Protocols /
Table of Contents: ... -- Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer Pipeline -- Synthesis and Evaluation of Novel Neamine-Nucleoside Conjugates as Potential Antibiotic Targets for Escherichia coli 16S Ribosomal RNA -- Electron...

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