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DNA Modifications [E-Book] : Methods and Protocols /
Table of Contents: ... -- Computational Analysis of DNA Modifications in Confocal Images -- Three-Dimensional Confocal Analysis of Chromosome Positioning Coupled with Immunofluorescence in Mouse Sperm Nuclei -- Analysis of 5-Hydroxymethyluracil Levels Using Flow Cytometry -- Hairpin-Bisulfite...

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Yeast Protocols [E-Book] /
Xiao, Wei
2021
Table of Contents: ... Assembly Using ReIN-Map -- Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly -- Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE -- Use YeastFab to Construct Genetic Parts and Multicomponent Pathways...

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Salmonella [E-Book] : Methods and Protocols /
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Capturing Chromosome Conformation [E-Book] : Methods and Protocols  /
Table of Contents: ...Capturing Chromosome Conformation -- The Chromosome Conformation Capture (3C) in Drosophila melanogaster -- 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture -- Analysis, Modeling, and Visualization of Chromosome...

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Parasite Genomics [E-Book] : Methods and Protocols  /
Table of Contents: ...Nanopore Long Read DNA Sequencing of Protozoan Parasites: Hybrid Genome Assembly of Trypanosoma cruzi -- Chromosomes Conformation Capture Coupled with Next Generation Sequencing (Hi-C) in Plasmodium falciparum -- Sequencing and Reconstructing Helminth...

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Germline Development in the Zebrafish [E-Book] : Methods and Protocols /
Table of Contents: ... CT Imaging of Zebrafish Gonads -- Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries -- Detection of the Polar Body after Fertilization -- Labelling the Micropylar Cell in Zebrafish Whole...

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Bifidobacteria [E-Book] : Methods and Protocols  /
Table of Contents: ...Methods for the Isolation and Recovery of Bifidobacteria -- Bifidobacterium Transformation -- Isolation of Chromosomal and Plasmid DNA from Bifidobacteria -- Assembly, Annotation, and Comparative Analysis of Bifidobacterial Genomes -- Site-directed...

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Computational Methods in Synthetic Biology [E-Book] /
Table of Contents: ... with Desired Functionalities -- A Deterministic Compartmental Modeling Framework for Disease Transmission -- Computer Aided Assembly and Verification of Synthetic Chromosomes -- Minimal Genome Design Algorithms using Whole-Cell Models -- Tn-Core: Functionally...

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Homologous Recombination [E-Book] : Methods and Protocols  /
Table of Contents: ... Electrophoresis and Southern Blotting -- Analysis of DNA Double-Strand Break Ends Resection and Single-Strand Annealing in S. pombe -- Quantifying DNA End Resection in Human Cells -- Genetic and Molecular approaches to Study Chromosomal Breakage at Secondary Structure...

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Epigenetic Reprogramming During Mouse Embryogenesis [E-Book] : Methods and Protocols /
Table of Contents: ... -- Investigating the Inner Cell Mass of the Mouse Blastocyst by Combined Immunofluorescence Staining and RNA Fluorescent In Situ Hybridization -- Mapping of Chromosome Territories by 3D-Chromosome Painting during Early Mouse Development -- Deciphering the Early Mouse...

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Bacterial Persistence [E-Book] : Methods and Protocols  /
Table of Contents: ... Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence -- Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods -- Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa -- The use of Experimental...

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Cell Cycle Oscillators [E-Book] : Methods and Protocols  /
Table of Contents: ... Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody -- Analyzing Centrioles and Cilia by Expansion Microscopy -- Analysis of Cell Cycle Progression in the Budding Yeast S. cerevisiae...

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Doubled Haploid Technology. Volume 1. General Topics, Alliaceae, Cereals [E-Book] /
Table of Contents: ... -- Methods for Chromosome Doubling -- Doubled Haploid Onion (Allium cepa L.) Production via In Vitro Gynogenesis -- In Vitro Gynogenesis in Leek (Allium ampeloprasum L.) -- Barley Isolated Microspore Culture -- Site-Directed Mutagenesis in Barley using RNA-GUided...

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Testicular Germ Cell Tumors : Methods and Protocols [E-Book] /
Table of Contents: ... -- Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors -- Germ Cell Tumor Cell Culture Techniques -- Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops -- Cultivation of Testicular...

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Cytoskeleton Dynamics [E-Book] : Methods and Protocols  /
Table of Contents: ... of Fluorescent Tubulin -- Functional Dissection of Mitosis using Immortalized Fibroblasts from the Indian Muntjac, A Placental Mammal with only Three Chromosomes -- Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes...

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MERS Coronavirus [E-Book] : Methods and Protocols  /
Table of Contents: ... of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein -- Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV -- Deducing the Crystal Structure of MERS-CoV Helicase -- Antigen...

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Plant Meiosis [E-Book] : Methods and Protocols  /
Table of Contents: ...A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis -- Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes -- How to Perform an Accurate Analysis of Metaphase I Chromosome Configurations in Autopolyploids of Arabidopsis...

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Trinucleotide Repeats [E-Book] : Methods and Protocols  /
Table of Contents: ... cerevisiae -- Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay -- Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site -- Experimental System to Study Instability of (CGG)n...

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Cereal Genomics [E-Book] : Methods and Protocols /
Table of Contents: ... -- Sequencing and Assembling Genomes and Chromosomes of Cereal Crops -- Cost-effective Profiling of Mutator Transposon Insertions in Maize by Next-generation Sequencing -- Relative Expression Analysis of target genes by using Reverse transcription-Quantitative PCR...

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Molecular Toxicology Protocols [E-Book] /
Table of Contents: ... -- Analysis of Telomere Length and Aberrations by Quantitative FISH -- Detection of Loss of Heterozygosity in Tk-Deficient Mutants from L5178Y Tk+/--3.7.2C Mouse Lymphoma Cells -- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation...

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