1
Human Naïve Pluripotent Stem Cells [E-Book] /
Table of Contents: ... Stem Cells using ATAC-seq -- Transcriptomic Analysis of Human Naïve and Primed Pluripotent Stem Cells -- Study of X Chromosome Activity Status in Human Naïve Pluripotent Stem Cells using RNA-FISH -- Flow Cytometry Analysis of Cell-Surface Markers to Identify...

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Plant Cell Division [E-Book] : Methods and Protocols  /
Table of Contents: ...Methods to Visualize the Actin Cytoskeleton during Plant Cell Division -- Immunolabeling of Nuclei/Chromosomes in Arabidopsis thaliana -- A Protocol for Genome-Wide Analysis of DNA Replication Timing in Intact Root Tips -- Assaying Cyclin-Dependent...

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Mitosis [E-Book] : Methods and Protocols  /
Table of Contents: ... Chambers for Arabidopsis Seedlings for Mitotic Studies -- Live Fluorescence Imaging of Chromosome Segregation in Cultured Cells -- Staining of the Actin Cytoskeleton during Cell Division in Budding Yeast and Mammalian Cells -- Synchronizing Mammalian Cells...

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Germline Development in the Zebrafish [E-Book] : Methods and Protocols /
Table of Contents: ... CT Imaging of Zebrafish Gonads -- Live and Time-lapse Imaging of Early Oogenesis and Meiotic Chromosomal Dynamics in Cultured Juvenile Zebrafish Ovaries -- Detection of the Polar Body after Fertilization -- Labelling the Micropylar Cell in Zebrafish Whole...

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Capturing Chromosome Conformation [E-Book] : Methods and Protocols  /
Table of Contents: ...Capturing Chromosome Conformation -- The Chromosome Conformation Capture (3C) in Drosophila melanogaster -- 4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture -- Analysis, Modeling, and Visualization of Chromosome...

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Cell Cycle Oscillators [E-Book] : Methods and Protocols  /
Table of Contents: ... Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody -- Analyzing Centrioles and Cilia by Expansion Microscopy -- Analysis of Cell Cycle Progression in the Budding Yeast S. cerevisiae...

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Testicular Germ Cell Tumors : Methods and Protocols [E-Book] /
Table of Contents: ... -- Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors -- Germ Cell Tumor Cell Culture Techniques -- Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops -- Cultivation of Testicular...

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Cytoskeleton Dynamics [E-Book] : Methods and Protocols  /
Table of Contents: ... of Fluorescent Tubulin -- Functional Dissection of Mitosis using Immortalized Fibroblasts from the Indian Muntjac, A Placental Mammal with only Three Chromosomes -- Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes...

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Mouse Oocyte Development [E-Book] : Methods and Protocols /
Table of Contents: ... -- Monitoring Microtubule Dynamics in the Mouse Egg using PhotoActivatableGFP-tubulin -- Photoactivation of Actin in Mouse Oocyte -- Laser Ablation of Microtubule-chromosome Attachment in Mouse Oocytes -- Micropipette Aspiration of Oocytes to Assess Cortical Tension....

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Molecular Motors [E-Book] : Methods and Protocols /
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Erythropoiesis [E-Book] : Methods and Protocols /
Table of Contents: ... of Erythroblast Enucleation -- High-Resolution Fluorescence Microscope Imaging of Erythroblast Structure -- Chromatin Immunoprecipitation (ChIP) with Erythroid Samples -- Chromosome Conformation Capture (3C and Higher) with Erythroid Samples -- Genome Editing...

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Cellular Heterogeneity [E-Book] : Methods and Protocols /
Table of Contents: ... Structural Chromosomal Aberrations -- FACS Isolation of Viable Cells in Different Cell Cycle Stages from Asynchronous Culture for RNA-Sequencing -- Measuring Nanoscale Chromatin Heterogeneity with Partial Wave Spectroscopic Microscopy....

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Cell Cycle Synchronization [E-Book] : Methods and Protocols /
Table of Contents: ... of Cells by Inhibitors of DNA Replication: Analysis by Flow Cytometry -- Chromosome Formation During Fertilization in Eggs of the Teleost Oryzias Latipes -- Flow Cytometry Analysis of Cell Cycle and Specific Cell Synchronization with Butyrate -- Chemically...

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Histones [E-Book] : Methods and Protocols /
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Fluorescence In Situ Hybridization (FISH) [E-Book] : Application Guide /
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The Mitotic Exit Network [E-Book] : Methods and Protocols /
Table of Contents: ... of the Mitotic Exit Network at Spindle Pole Bodies -- Evaluation of the Nucleolar Localization of the RENT Complex to Ribosomal DNA by Chromatin Immunoprecipitation Assays -- Analysis of the Functionality of the Mitotic Checkpoints -- Cdc14 and Chromosome Condensation...

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Cohesin and Condensin [E-Book] : Methods and Protocols /
Table of Contents: ...A Sister Chromatid Cohesion Assay Using Xenopus Egg Extracts -- An In Vitro Assay for Monitoring Topological DNA Entrapment by the Chromosomal Cohesin Complex -- Biochemical and Functional Assays of Human Cohesin-Releasing Factor Wapl -- Detection...

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The Nucleolus [E-Book] : Methods and Protocols /
Table of Contents: ... of Chromosome Domain – Nucleolus Associations in S. Cerevisiae -- Quantitative Immunofluorescence Analysis of Nucleolus-Associated Chromatin -- Visualization of The Nucleolus in Living Cells with Cell-Penetrating Fluorescent Peptides -- Purification of S. Cerevisiae...

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Oogenesis [E-Book] : Methods and Protocols /
Table of Contents: ... Electron Microscopy of Zebrafish Oocytes -- Cytoskeleton and Cytoskeleton-Bound RNA Visualization in Frog and Insect Oocytes -- RNA FISH, DNA FISH and Chromosome Painting of Chicken Oocytes -- Preparation of Cell Lysate from Mouse Oocytes for Western Blotting...

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Life Sciences and Radiation [E-Book] : Accomplishments and Future Directions /
Table of Contents: ... of Radiation-Induced Signalling -- Cytogenetics -- Molecular Genetic Mechanisms of Radiocarcinogenesis and Predictive Markers for Radioresistance in Tumors — Present Work and Future Perspectives — -- FISH and CHIPs: Colorful Clues to Radiation-Induced Chromosomal...

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